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目的探讨大肠癌患者围手术期血清热休克蛋白60(HSP60)和基质金属蛋白酶-9(MMP-9)水平的变化及其临床意义。方法采用ELISA法分别测定76例大肠癌患者手术前后血清HSP60和MMP-9的含量,并与大肠良性疾病组和健康对照组比较分析。结果大肠癌组治疗前血清HSP60和MMP-9均增高,与大肠良性疾病组和健康对照组比较有显著性差异(P<0.01);且与淋巴结转移和肿瘤临床Duke’s分期呈明显相关性。行根治性手术组术后血清HSP60和MMP-9均有显著性降低(P<0.05),而姑息性手术组血清HSP60和MMP-9则无显著性降低(P>0.05)。结论大肠癌患者血清HSP60和MMP-9的水平明显升高,检测大肠癌患者血清HSP60和MMP-9的变化,有助于估计患者的病情。
Objective To investigate the changes of serum heat shock protein 60 (HSP60) and matrix metalloproteinase-9 (MMP-9) levels in patients with colorectal cancer and their clinical significance. Methods The levels of serum HSP60 and MMP-9 in 76 patients with colorectal cancer before and after operation were determined by ELISA. The levels of serum HSP60 and MMP-9 were compared with benign disease group and healthy control group. Results The serum levels of HSP60 and MMP-9 in colorectal cancer group before treatment were significantly higher than those in benign colorectal disease group and healthy control group (P <0.01), and significantly correlated with lymph node metastasis and clinical Duke’s stage. The serum levels of HSP60 and MMP-9 in the radical operation group were significantly decreased (P <0.05), while the serum levels of HSP60 and MMP-9 in the palliative operation group were not significantly reduced (P> 0.05). Conclusions Serum levels of HSP60 and MMP-9 in patients with colorectal cancer are significantly increased. Detecting the changes of serum HSP60 and MMP-9 in patients with colorectal cancer is helpful to evaluate the patient’s condition.