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选用高效选择性 i NOS抑制剂 1 4 0 0 w治疗 96只雄性大鼠提睾肌缺血再灌注损伤 ,观测去神经完全缺血 3小时大鼠提睾肌再灌注 90分钟期间滋养动脉血管管径恢复率及血液流动率变化 ,探讨选择性抑制i NOS对骨骼肌缺血再灌注损伤的治疗作用及机制。结果发现 ,大鼠提睾肌去滋养神经后完全缺血 3小时 ,随着血循环的重建 ,PBS组“无复流”现象的发生率为 2 0 % ,70 %的实验动物在灌注半小时内血管管径及血流恢复率仅为缺血前的 60～ 80 % ;而 1 4 0 0 W组 ,“无复流”现象降低至 5% ,90 %的实验动物在灌注 1 0分钟后血管管径及血流速率均增至缺血前的 1 1 0～ 1 2 0 % ,肌肉表面光洁度基本正常。此外 ,注射 PBS与 1 4 0 0 W后 ,连续测量大鼠的血压、呼吸、脉搏三大生命体征 2小时 ,生命体征平稳。从而表明 ,1 4 0 0 W通过选择性高效抑制 i N-OS,能够减轻或消除骨骼肌缺血再灌注损伤 ,且能平衡保持三大生命体征
Selection of highly selective i NOS inhibitor 1 4 0 0 w treatment of 96 male rats with cremaster muscle ischemia-reperfusion injury observed 3 hours after complete denervation of the denervation of the rat cremaster muscle 90 minutes during reperfusion nourish the arterial blood vessels Diameter recovery rate and blood flow rate changes, to explore the selective inhibition of i NOS on skeletal muscle ischemia-reperfusion injury and its mechanism. The results showed that the rat cremasteric muscle to nourish nerves completely ischemia for 3 hours, with the reconstruction of blood circulation, PBS group “no-reflow” phenomenon was 20%, 70% of experimental animals within half an hour of perfusion Blood vessel diameter and blood flow recovery rates were only 60-80% of pre-ischemic ones; however, the “no-reflow” phenomenon was reduced to 5% in 14,000 W and 90% in experimental animals The diameter and blood flow rate increased to 110 ~ 120% before ischemia, and the muscle surface smoothness was basically normal. In addition, after PBS and 1400 W were injected, the three vital signs of respiration and pulse were measured continuously for 2 hours, and the vital signs were stable. Thus, 1 4 0 0 W can reduce or eliminate skeletal muscle ischemia-reperfusion injury by selectively and efficiently inhibiting i N-OS, and can maintain and maintain the three vital signs