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目的:观察肺炎衣原体(C.pn)感染对血管平滑肌细胞(VSMC)迁移的影响,探讨TLR2-PI3K/Akt信号转导通路在C.pn感染诱导VSMC迁移中的作用。方法:组织贴块法行大鼠原代VSMC培养;免疫荧光法确认C.pn成功感染VSMC;Wound-healing assay和Transwell assay观察C.pn感染对VSMC迁移能力的
Objective: To observe the effect of C.pn infection on the migration of vascular smooth muscle cells (VSMCs) and to explore the role of TLR2-PI3K / Akt signaling pathway in the migration of VSMCs induced by C.pn infection. Methods: The rat primary VSMCs were cultured by tissue block method, VSMCs were successfully infected by C.pn by immunofluorescence, Wound-healing assay and Transwell assay were used to observe the migration of VSMCs