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目的:探讨产前孕鼠使用地塞米松不同剂量及次数对早产仔鼠脑发育的影响。方法:孕大鼠分为5组,对照组、地塞米松高剂量组(0.8mg/kg)和低剂量组(0.2mg/kg),地塞米松多次使用(3次)及单次使用组,于孕19天处死大鼠,取仔鼠称体重及脑重,免疫组化检测脑神经元特异性烯醇化酶(NSE)表达情况。结果:地塞米松处理可降低早产仔鼠体重及脑重,脑皮质及海马区NSE表达降低,且大剂量多次使用比低剂量单次使用对脑组织的损伤更大。结论:产前使用地塞米松可损伤早产仔鼠脑发育,临床应考虑使用低毒性的糖皮质激素替代地塞米松。
Objective: To investigate the effects of dexamethasone on the brain development of premature pups in prenatal pregnant rats with different doses and times. Methods: The pregnant rats were divided into 5 groups: control group, dexamethasone high dose group (0.8mg / kg) and low dose group (0.2mg / kg), dexamethasone multiple use The rats were sacrificed on day 19 of pregnancy. The body weight and brain weight of the rats were measured. The expression of neuron specific enolase (NSE) was detected by immunohistochemistry. Results: Dexamethasone treatment could reduce the body weight and brain NSE expression in brain, cerebral cortex and hippocampus of premature offspring rats, and the high dose repeated use had more damage to brain tissue than the low dose single use. Conclusion: Prenatal use of dexamethasone can damage brain development of premature newborn rats, and should consider the use of low toxicity glucocorticoid instead of dexamethasone.