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从病死的黄沙鳖分离出3株细菌,通过对菌株的形态、培养特征、生理生化特征研究以及16SrDNA序列分析,结果表明,3株分离菌均为乳球菌属的格氏乳球菌。对健康白兔和鳖的人工感染试验,表明所分离鉴定的格氏乳球菌对鳖有致病性;药敏试验结果显示,在供试10种抗菌素中,对头孢哌酮等4种药物高敏,对恩诺沙星等4种药物中敏,对多粘霉素B等2种药物耐药。
Three strains of bacteria were isolated from the dead yellow sand turtle. The morphology, culture characteristics, physiological and biochemical characteristics and 16S rDNA sequence of the strains were analyzed. The results showed that all the three isolates were Lactococcus. Artificial infection experiments on healthy white rabbits and soft-shelled turtles showed that L. griseofuliensis isolated and identified had pathogenicity to soft-shelled turtles. The results of susceptibility test showed that among the ten antibiotics tested, the sensitivity to four drugs such as cefoperazone was high , Four kinds of drugs such as enrofloxacin sensitive, polymyxin B and other two kinds of drug resistance.