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目的进一步完善兔VX2肝种植肿瘤模型的制作,比较种植肿瘤的CT、MRI及DSA影像表现,评价各影像检查对瘤体显示的优越性。方法实验动物为新西兰大白兔(前期实验12只,后期实验24只),采用动物后肢皮下注射接种传代保存瘤种。模型前期制作采用包埋法接种．后期采用针头注入法接种于兔肝左叶,并于术前1 d及术后1、3 d、1、2周检查肝肾功能的动态变化情况。接种2周后行CT、MR成像,随后行经股动脉肝动脉超选择DSA及纳米磁性粒子介入治疗。结果前期实验肿瘤种植成功率66．7％,后期实验种植成功率100％。术后ALT、AST。有一过性增高,无统计学意义:BUN、Cr无明显变化。术后CT平扫肿瘤为略低密度病灶,强化不明显,境界清晰;MR平扫呈长T1、长T2信号;EPI序列T2WI呈略高信号影;DWI成像见高亮信号影,显示清晰;介入术中DSA造影可见肝左叶孤立的丰富血管肿瘤,供血动脉增粗,经超选择注入纳米磁性粒子,肿瘤内密度明显增高。结论兔VX2肝种植肿瘤模型是介入治疗实验研究理想的动物模型。VX2瘤粒针头注入法较包埋法成功率更高,其建立过程对兔肝肾功能无明显影响。CT平扫增强,MR平扫,EPI序列及DWI成像对于肿瘤的影像评价互有优势,EPI及DWI成像可更灵敏地显示肿瘤,DSA可清晰地显示肿瘤的供血及肝内有无转移。
Objective To further improve the production of rabbit VX2 liver tumor model and compare the CT, MRI and DSA imaging performance of the implanted tumor to evaluate the superiority of each image examination on tumor display. Methods The experimental animals were New Zealand white rabbits (12 in the early stage and 24 in the late stage). The tumor was subcultured and subcutaneously injected into the hind limbs of animals. Model pre-production by embedding. In the later stage, the needle was implanted into the left lobe of rabbit liver, and the dynamic changes of liver and kidney function were examined on the first day before operation and on the 1st, 3rd and 1st, 2nd and 2nd after operation. Two weeks after inoculation, CT and MR imaging were performed, followed by DSA and nano-magnetic particle intervention via hepatic artery of femoral artery. Results The successful rate of early tumor implantation was 66.7% and the success rate of late experiment was 100%. Postoperative ALT, AST. There was a transient increase, no statistical significance: BUN, Cr no significant change. Postoperative CT scan of the tumor was a slightly lower density lesion, the enhancement was not obvious, the realm clear; MR plain scan showed long T1, long T2 signal; EPI T2WI slightly higher signal; DWI imaging see the bright signal, showing clear; DSA angiography can be seen in the left hepatic lobe isolated rich vascular tumor, feeding artery thickening, by superselective injection of nano-magnetic particles, tumor density was significantly increased. Conclusion The rabbit model of VX2 liver tumor is an ideal animal model for the experimental study of interventional therapy. The success rate of VX2 tumor needle injection method was higher than embedding method, and its establishment process had no significant effect on liver and kidney function in rabbits. CT scan enhancement, MR plain scan, EPI sequence and DWI imaging each have advantages in imaging evaluation of tumors. EPI and DWI imaging can show tumor more sensitively. DSA can clearly show tumor blood supply and intrahepatic metastasis.