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目的：了解咖啡因对胚胎及新生时期生殖细胞合成ＤＮＡ的影响及其机制。方法：采用低（０３ｍｍｏｌ／Ｌ）、中（０６ｍｍｏｌ／Ｌ）、高（１２ｍｍｏｌ／Ｌ）浓度咖啡因体外培养ＳＤ孕１８ｄ胎鼠、０ｄ及４ｄ乳鼠睾丸组织块，培养时间分别为１、２、３周，用放射自显影、计算机图像分析的方法观察咖啡因对生殖细胞摄取［３Ｈ］－ＴｄＲ及ＤＮＡ含量的影响。结果：（１）１８ｄ胎鼠、０ｄ乳鼠、４ｄ乳鼠睾丸培养组织内生殖细胞受咖啡因影响依次增加。（２）浓度越高、培养时间越长，咖啡因对生殖细胞影响越明显。（３）生殖细胞数量的减少往往伴随ＤＮＡ含量和［３Ｈ］－ＴｄＲ摄取的降低。结论：高浓度咖啡因长时间培养后使生殖细胞数量减少可能与干扰细胞摄取ＤＮＡ前体物质，降低细胞内ＤＮＡ含量有关。
Objective: To investigate the effect of caffeine on the DNA synthesis of germ cells in embryonic and neonatal period and its mechanism. Methods: The testes of SD pregnant 18 d, 0 d and 4 d SD rats were cultured in low (0.3 mmol / L), (0.6 mmol / L) and high (1.2 mmol / L) 1, 2, and 3 weeks respectively. The effects of caffeine on the uptake of [3H] -TdR and DNA by germ cells were observed by autoradiography and computer image analysis. Results: (1) The germ cells in testis tissue of 18 d fetuses, 0 d suckling rats and 4 d suckling rats were increased by caffeine. (2) The higher the concentration, the longer the culture time, the more obvious the effect of caffeine on germ cells. (3) The decrease in the number of germ cells is often accompanied by a decrease in DNA content and [3H] -TdR uptake. Conclusion: The decrease of germ cell number after prolonged incubation of high concentration caffeine may be related to interfering with the uptake of DNA precursors and reducing the intracellular DNA content.