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目的 :探讨腺苷和ATP对肾脏缺血再灌注损伤的保护作用机制并进行相互比较。方法 :将大鼠随机分为对照组 (CON)、缺血再灌注组 (IR)、给予ATP后缺血再灌注组 (ATP)和给予腺苷后缺血再灌注组 (AD) ,分别观察各组中在再灌注 0h、2h和 6h的Na+和K+-ATP酶和Ca2 +-ATP酶活性的变化 ,以及组织病理变化。结果 :病理学组织学肾小管评分 ,腺苷组和CON组之间无显著性差异 (P >0 .0 5 ) ,但两组均明显低于IR组和ATP组 (P <0 .0 5或P <0 .0 1) ,IR组和ATP组之间无显著差异 (P >0 .0 5 )。Na+,K+-ATP酶和Ca2 +-ATP酶的活性 ,在腺苷组和CON组之间无显著性差别 ,(P >0 .0 5 ) ,但两组均明显高于IR组和ATP组 (P <0 .0 1) ,而IR组和ATP组之间无显著性差异 (P >0 .0 5 )。结论 :腺苷对大鼠肾脏缺血再灌注损伤没有保护作用 ,ATP对大鼠肾脏缺血再灌注损伤有保护作用。
Objective: To investigate the protective effect of adenosine and ATP on renal ischemia-reperfusion injury and to compare with each other. Methods: The rats were randomly divided into control group (CON), ischemia / reperfusion group (IR), ATP group and adenosine group after ischemia / reperfusion (AD) The changes of Na +, K + -ATPase and Ca2 + -ATPase activities at 0h, 2h and 6h after reperfusion in each group, as well as histopathological changes. Results: There was no significant difference between tubulointerstitial histopathology and adenosine group (P> 0.05), but both groups were significantly lower than IR group and ATP group (P <0.05) Or P <0.01), there was no significant difference between IR group and ATP group (P> 0.05). The activity of Na +, K + -ATPase and Ca2 + -ATPase showed no significant difference between adenosine group and CON group (P> 0.05), but both groups were significantly higher than IR group and ATP group (P <0.01), but there was no significant difference between IR group and ATP group (P> 0.05). CONCLUSION: Adenosine has no protective effect on renal ischemia-reperfusion injury in rats, and ATP has a protective effect on kidney ischemia-reperfusion injury in rats.