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目的研究己烯雌酚对 T细胞肿瘤的细胞凋亡诱导作用 ,以及调亡过程中转录因子 Oct- 1的表达。方法采用DNA梯形片段化 ,荧光染色流式细胞技术分析细胞凋亡 ,以电泳泳动度迁移率法 (EMSA)检测 Oct- 1的表达。结果己烯雌酚 (5 .0 mg/L )可诱导 T细胞出现细胞凋亡 ,并有典型的 DNA梯形片段化 ,10μg己烯雌酚诱导 12 h后 ,凋亡过程中细胞数达 30 %以上。己烯雌酚诱导 T细胞凋亡过程与 Oct- 1转录因子的表达有关。结论己烯雌酚可诱导 T细胞肿瘤凋亡 ,并与转录因子 Oct- 1的表达有关。
Objective To study the apoptosis-inducing effect of diethylstilbestrol on T cell tumors and the expression of transcription factor Oct-1 in apoptosis. Methods DNA ladder was used for DNA fragmentation. Flow cytometry was used to analyze apoptosis. The expression of Oct-1 was detected by electrophoretic mobility shift assay (EMSA). RESULTS: Diethylstilbestrol (5.0 mg/L) could induce apoptosis of T cells and typical DNA fragmentation. After 12 h of induction with 10 μg of diethylstilbestrol, the number of cells during apoptosis was more than 30%. The process of apoptosis induced by diethylstilbestrol is related to the expression of Oct-1 transcription factor. Conclusion Diethylstilbestrol can induce T cell tumor apoptosis and is related to the expression of transcription factor Oct-1.