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目的:探讨黄芪甲苷对人骨髓间充质干细胞体外增殖及细胞因子表达的影响。方法:采用Percoll密度离心法和贴壁法分离纯化hBMSC;流式细胞术检测hBMSC表面标志;MTT法检测不同浓度黄芪甲苷干预72 h后hBMSC的增殖情况;Real-time PCR法检测经黄芪甲苷干预后hBMSC对SCF、VEGF、SDF-1、GM-CSF mRNA的表达水平。结果:成功分离培养出hBMSC;不同浓度(20、40、80、160、320 mg/mL)的黄芪甲苷可促进hBMSC增殖(P<0.05),其中160 mg/mL组促增殖最明显;黄芪甲苷可促进hBMSC对SCF、VEGF、SDF-1 mRNA的表达,而GM-CSF mRNA表达无明显变化。结论:黄芪甲苷可促进hBMSC体外增殖,可能与其促进hBMSC对SCF、VEGF、SDF-1 mRNA表达有关。
Objective: To investigate the effects of Astragaloside IV on proliferation and cytokine expression of human bone marrow mesenchymal stem cells in vitro. Methods: The hBMSCs were isolated and purified by Percoll density centrifugation and adherent method. The surface markers of hBMSCs were detected by flow cytometry. The proliferation of hBMSCs was detected by MTT assay at 72 h. Real-time PCR After intervention of hBMSC, the expression of SCF, VEGF, SDF-1 and GM-CSF mRNA were detected. Results: The hBMSC was successfully isolated and cultured. Astragaloside at different concentrations (20, 40, 80, 160 and 320 mg / mL) could promote the proliferation of hBMSCs (P < Cytosine can promote the expression of SCF, VEGF and SDF-1 mRNA in hBMSCs, while the expression of GM-CSF mRNA did not change significantly. Conclusion: Astragaloside IV can promote the proliferation of hBMSC in vitro, which may be related to the promotion of hBMSC on the expression of SCF, VEGF and SDF-1 mRNA.