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目的探讨爆震后豚鼠耳蜗听阈明显升高时螺旋神经节细胞超微结构改变及其中睫状神经营养因子表达的变化。方法复制爆震致聋豚鼠模型,不同时段测ABR阈值,取耳蜗做病理,观察耳蜗螺旋神经节细胞数量和超微结构的变化；用免疫组化检测耳蜗螺旋神经节细胞内CNTF的表达。结果爆震组21d的耳蜗螺旋神经节细胞在二下组为(25．00±7．16)个,而正常对照组为(52．00±5．32)个。以上数据组间统计学差异有显著意义。豚鼠螺旋神经节细胞透射电镜观察见爆震组震后3d出现线粒体明显肿胀,线粒体嵴断裂；21d后线粒体数量明显减少,且有畸形；经爆震声暴露后21d的豚鼠耳蜗中轴切片上CNTF阳性反应细胞数量减少,而且染色分布不均匀。结论爆震对耳蜗螺旋神经节细胞的数量和超微结构均有明显影响,同时在早期干扰螺旋神经节细胞内CNTF的表达。
Objective To investigate the changes of the ultrastructure of spiral ganglion cells and the changes of ciliary neurotrophic factor in guinea pig cochlea after detonation. Methods Deaf guinea pigs were induced by detonation. The ABR threshold was measured at different time points. The cochlea were used for the pathological changes. The number and ultrastructure of spiral ganglion cells were observed. The expression of CNTF in the cochlear spiral ganglion cells was detected by immunohistochemistry. Results The number of cochlear spiral ganglion cells in the second group was (25.00 ± 7.16) in the detonation group and (52.00 ± 5.32) in the normal control group. Statistical differences between the above data groups have significant significance. The mitochondria were obviously swollen and the mitochondrial cristae were broken on the guinea pig spiral ganglion cells after transmission electron microscope. The number of mitochondria was significantly reduced and deformity appeared on the 21th day after detonation. The cochlear CNTF The number of positive cells decreased, and the dyeing distribution was not uniform. Conclusion Detonation has a significant effect on the number and ultrastructure of spiral ganglion cells in the cochlea, and at the same time it interferes with the expression of CNTF in the spiral ganglion cells at early stage.