论文部分内容阅读
目的 研究抗人结肠癌MAb检测血清及粪液中癌相关抗原的价值。 方法 采用抗人结肠癌单抗SC_(3A)和SC_6检测47例结直肠癌、33例结直肠腺瘤、15例增生性息肉、14例炎性息肉及60例正常人群的血清和粪便中癌相关抗原。经ELISA双抗体夹心法检测。 结果 血清和粪便阳性率在结直肠癌组分别为66.0％和72.3％;腺瘤组为63.6％和60.6％;增生性息肉组为35.7％和57.1％;炎性息肉组为40.0％和33.3％;而正常对照组仅为11.7％和13.3％。45例大肠癌Dukes分期进行检测,早期癌粪便检测的阳性率为72.2％,而血清为50.0％。 结论 ELISA法检测不仅对大肠癌及癌前病变具有较好的敏感性,而且对早期癌及癌前期病变检测较血清学检测意义更大。此外,随腺瘤不典型增生程度加重而检测阳性率增高。粪便中检测大肠癌相关抗原,方法简便,取材容易,具较好敏感性,对大规模人群普查大肠癌具有一定的应用价值。
Objective To study the value of anti-human colon cancer MAb for the detection of cancer-associated antigen in serum and feces. Methods Anti-human colon cancer monoclonal antibodies SC_(3A) and SC_6 were used to detect the serum and fecal metastases in 47 cases of colorectal cancer, 33 cases of colorectal adenoma, 15 cases of hyperplastic polyp, 14 cases of inflammatory polyps, and 60 cases of normal people. Related antigens. The ELISA double antibody sandwich assay. Results The positive rates of serum and feces were 66.0% and 72.3% in the colorectal cancer group, 63.6% and 60.6% in the adenoma group, 35.7% and 57.1% in the hyperplastic polyp group, and 40.0% and 33.3% in the inflammatory polyp group, respectively. However, the normal control group was only 11.7% and 13.3%. Forty-seven cases of colorectal cancer Dukes were detected by stages. The positive rate of early stage cancer stool tests was 72.2%, while that of serum was 50.0%. Conclusions The ELISA assay has not only good sensitivity to colorectal cancer and precancerous lesions, but also has a greater significance for the detection of early cancer and precancerous lesions than serological detection. In addition, the positive rate of detection was increased with aggravation of adenoma hyperplasia. Detection of colorectal cancer-associated antigens in feces is simple, easy to obtain, and has good sensitivity. It has certain application value for large-scale population screening for colorectal cancer.