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最近研究发现,氧化应激和氧自由基的产生既是癫痫发作的原因也是癫痫发作导致继发损伤的原因。鉴于硒和硒蛋白可对抗氧化损伤并提高神经细胞的存活,研究对比癫痫和正常儿童的血清硒水平及红细胞谷胱甘肽过氧化物酶活性。对照研究居住于同一地域处于同一年龄段的53个癫痫病儿童和57个正常儿童。血清硒水平和红细胞谷胱甘肽过氧化物酶活性分别用原子吸收分光光度仪和Gayman标准谷胱甘肽过氧化物酶检测盒来测定。结果显示,患者和正常个体血清硒水平分别为(72.90±22.20)μg/L和(86.00±15.00)μg/L。红细胞谷胱甘肽过氧化物酶活性数值在患者和正常人分别为(0.441±0.264)nmol/(min.L)和(0.801±0.267)nmol/(min.L)。统计分析发现,患者硒和红细胞谷胱甘肽过氧化物酶水平较对照组显著降低。研究认为,患者血清硒和红细胞谷胱甘肽过氧化物酶活性较正常人低下,这一结果支持硒和谷胱甘肽过氧化物酶在癫痫病因中扮演重要角色这一观点。然而,红细胞谷胱甘肽过氧化物酶活性数值在硒缺乏个体并不能作为一个灵敏而特异指标来反映硒水平,所以研究者用硒检测来替代红细胞谷胱甘肽过氧化物酶活性测定。
Recent studies have found that the generation of oxidative stress and oxygen free radicals are both the cause of seizures and the causes of secondary damage caused by seizures. Since selenium and selenoprotein are able to resist oxidative damage and increase the survival of nerve cells, selenium level and erythrocyte glutathione peroxidase activity in epileptic and normal children are compared. The controlled study lived in 53 epilepsy children and 57 normal children in the same age group. Serum selenium levels and erythrocyte glutathione peroxidase activity were determined by atomic absorption spectrophotometry and Gayman’s standard glutathione peroxidase kit, respectively. The results showed that serum selenium levels in patients and normal individuals were (72.90 ± 22.20) μg / L and (86.00 ± 15.00) μg / L, respectively. Erythrocyte glutathione peroxidase activity was (0.441 ± 0.264) nmol / (min.L) and (0.801 ± 0.267) nmol / (min.L) in patients and normal controls, respectively. Statistical analysis found that patients with selenium and erythrocyte glutathione peroxidase levels were significantly lower than the control group. Studies suggest that patients with serum selenium and erythrocyte glutathione peroxidase activity lower than normal, a result that supports the selenium and glutathione peroxidase in the etiology of epilepsy play an important role in this view. However, the activity of erythrocyte glutathione peroxidase activity in selenium deficient individuals does not reflect selenium level as a sensitive and specific indicator, so researchers replaced selenium detection with glutathione peroxidase activity assay.