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对已转入rolB、rolC基因741杨株系进行分子检测,并对其根系生长状况、苗高、叶绿素a和b、叶绿素荧光以及内源激素等指标进行测定。结果表明,转rolB、rolC基因741杨各株系发根率、发根量均高于未转基因对照。转rolB基因741杨株系苗高与对照无显著差异,叶绿素a、b含量上升,Fv/Fm、PI值下降,IAA、ZT含量上升,ABA含量下降。转rolC基因741杨各株系顶端优势减弱,植株矮化,叶绿素a、b含量降低,Fv/Fm、PI值下降,IAA、ZT、ABA含量上升。总体比较,转rolB基因741杨株系生根率,叶绿素a、b含量,PI值、ZT和IAA含量均高于转rolC基因741杨株系。
The transgenic lines of rol1 gene and rolC gene 741 were sequenced and their root growth status, seedling height, chlorophyll a and b, chlorophyll fluorescence and endogenous hormones were measured. The results showed that the hairiness rate and hairy root yield of each line of transgenic poplar rolB and rolC gene were higher than those of non-transgenic control. There was no significant difference in the seedling height between the transgenic lines 741 and the rolB gene. The content of chlorophyll a, b, Fv / Fm and PI decreased, while the content of IAA and ZT increased and the content of ABA decreased. The top advantages of transgenic lines 741 were weakened, plants dwarfed, chlorophyll a, b contents decreased, Fv / Fm and PI decreased, IAA, ZT and ABA contents increased. Overall, the rooting rate, chlorophyll a, b content, PI value, ZT and IAA content of rolB 741 transgenic lines were all higher than those of the transgenic rolu 741 poplar lines.