论文部分内容阅读
目的比较荧光定量PCR法和分离培养法在检测小肠结肠炎耶尔森菌中特异性和灵敏度的差异。方法从686份婴幼儿腹泻患儿的粪便标本中提取细菌基因组DNA,在进行小肠结肠炎耶尔森菌保守基因fox A和黏附侵袭位点基因ail的实时荧光定量PCR检测的同时,也按常规方法进行菌株分离培养,并对2种方法的检出结果进行比较分析。结果686份标本中,分离培养法检测结果为阳性4份,阴性682份;荧光定量PCR法检测结果为阳性5份,阴性681份。荧光定量PCR法检出的5份阳性标本中,分离培养法结果为4份阳性,1份阴性。结论采用fox A联合ail基因的荧光定量PCR法特异性强,灵敏度高,且操作简便快速,检测成本低,对于监测小肠结肠炎耶尔森菌有重要意义。
Objective To compare the differences of specificity and sensitivity in the detection of Yersinia enterocolitica by fluorescence quantitative PCR and isolation culture. Methods Bacterial genomic DNA was extracted from stool samples from 686 infants with diarrhea in infants. The real-time PCR detection of Yersinia enterocolitica conserved gene fox A and adhesion site ail was performed. Methods The strains were isolated and cultured, and the detection results of the two methods were compared. Results Among the 686 specimens, 4 were positive and 682 were negative. The results of fluorescence quantitative PCR were positive 5 and negative 681. Among the five positive samples detected by fluorescence quantitative PCR, four were positive and one was negative. Conclusion The fluorescence quantitative PCR method using fox A combined with ail gene has high specificity, high sensitivity, simple and rapid operation, low detection cost and is of great significance for monitoring Yersinia enterocolitica.