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S-a, S-b and S-c are three loci for F1 pollen sterility in cultivated rice (Oryza sativa L.). Taichung 65 (T65) is all S j/S j at these three loci, while its F1 pollen sterile near-isogenic lines, TISL2 (S-b), TISL4 (S-a) and TISL5 (S-c) is Si/Si according to their respective sterility locus. Using SSR molecular marker to detect the segregation of the allele S i and S j in pollen calli population induced from different hybrid F1, which have different pollen sterility locus, showed that the segregation of allele S i and S j was distorted. The distorted direction of pollen calli population in vitro was not the same as F2 population in vivo. The quantities of pollen callus carrying S j were much more than that of carrying S i at S-a and S-c locus, the ratio of S i and Sj were 1:4.81 and 1:1.96 respectively. But the opposite tendency was observed at S-b locus, the ratio of S i and S j being 1:0.35. At the same time, all these results were undisturbed by either culture medium or culture period.
Sa, Sb and Sc are three loci for F1 pollen sterility in cultivated rice (Oryza sativa L.). Taichung 65 (T65) is all S j / S j in these three loci while its F1 pollen sterile near-isogenic lines, TISL2 (Sb), TISL4 (Sa) and TISL5 (Sc) is Si / Si according to their respective sterility locus. Using SSR molecular marker to detect the segregation of the allele Si and Sj in pollen calli population induced from different hybrid Fl, which have different pollen sterility locus, showed that the segregation of allele S i and S j was distorted. The distorted direction of pollen calli population in vitro was not the same as F2 population in vivo. The quantities of pollen callus carrying S j were much more than that of carrying S i at Sa and Sc locus, the ratio of S i and S j were 1: 4.81 and 1: 1.96 respectively. But the opposite tendency was observed at Sb locus, the ratio of S i and S j being 1 : 0.35. At the same time, all these results were undisturbed by either culture medium or culture per iod.