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目的:探讨根皮素对人肝癌Hep G2细胞增殖和凋亡的影响。方法:MTT法检测不同浓度(30、40、50μg/m L)根皮素对肝癌Hep G2细胞增殖的抑制作用,流式细胞技术检测根皮素对Hep G2细胞凋亡的影响,ELISA法检测不同浓度根皮素干预后细胞中Bcl-2和Bax表达的变化,Western blot法检测30μg/m L根皮素在8,16,24小时后检测p-AKT蛋白表达情况。结果:30、40和50μg/m L的根皮素对肝癌Hep G2细胞的增殖均有抑制作用(P<0.01),同时,30、40和50μg/m L的根皮素在24小时后可诱导Hep G2细胞发生早期凋亡,凋亡率分别为0.1321±0.0224,0.2607±0.0457,0.3712±0.0884(P<0.01);另外,30、40和50μg/m L的根皮素作用24小时后细胞内Bcl-2表达降低,Bax表达增高(P<0.01);最后,30μg/m L根皮素可以明显减少p-AKT表达量,这种作用呈现时间依赖性。结论:根皮素能够抑制肝癌Hep G2细胞的增殖和促进细胞凋亡。
Objective: To investigate the effects of phloretin on the proliferation and apoptosis of Hep G2 cells. Methods: MTT assay was used to detect the inhibitory effects of phloretin on the proliferation of Hep G2 cells. Flow cytometry was used to detect the effects of phloretin on the apoptosis of Hep G2 cells. ELISA was used to detect the apoptosis of Hep G2 cells. The changes of Bcl-2 and Bax expression in different concentrations of phloretin were detected by Western blot. The expression of p-AKT protein was detected after 8, 16 and 24 hours. RESULTS: Phloretin at 30, 40 and 50 μg / mL had inhibitory effects on the proliferation of Hep G2 cells (P <0.01). Phloretin at 30, 40 and 50 μg / mL after 24 h The apoptosis rate of Hep G2 cells was 0.1321 ± 0.0224,0.2607 ± 0.0457 and 0.3712 ± 0.0884 respectively (P <0.01). In addition, after 24 hours, the cells were exposed to 30, 40 and 50 μg / Bcl-2 expression was decreased and Bax expression was increased (P <0.01). Finally, 30 μg / m L phloretin could significantly reduce the expression of p-AKT, which showed a time-dependent manner. Conclusion: Phloretin can inhibit Hep G2 cell proliferation and promote apoptosis.