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目的 探讨再灌注期吸入异氟醚对不同程度全脑缺血再灌注损伤的保护效应及其机制。方法 将42只雄性 SD 大鼠随机分为假手术组(n=6)、复苏组(n=18)及对照组(n=18)，后两组又分为缺血10min、15min、20min 三个时间点，每个时间点6只大鼠，建立大鼠清醒全脑缺血模型。复苏组于再灌注开始后立即吸入1.4%异氟醚30min 进行复苏。收集清醒、缺血及再灌注后微透析标本，测定谷氨酸递质浓度。并进行运动功能双盲评定。双盲记数海马 CA1区核完整的锥体细胞及凋亡细胞的百分率。结果 与对照组相比，复苏组缺血再灌注早期海马组织谷氨酸递质浓度降低(P<0.05)；全脑缺血15min 复苏组的运动功能评分升高(P<0.05)；全脑缺血10、15、20min 对照组和复苏组海马神经细胞凋亡率均低于假手术组(P<0.05)；全脑缺血10、15min 复苏组海马 CA1区神经细胞凋亡率降低(P<0.05)。结论 再灌注早期吸入异氟醚具有脑保护作用，其机制与促进脑缺血期间过度释放的谷氨酸递质于再灌注期的吸收有关。
Objective To investigate the protective effect and mechanism of inhalation of isoflurane on cerebral ischemia-reperfusion injury with different degrees of reperfusion. Methods 42 male SD rats were randomly divided into sham operation group (n = 6), resuscitation group (n = 18) and control group (n = 18). The latter two groups were further divided into three groups: ischemia 10min, A time point, each time point 6 rats, the establishment of rat model of global cerebral ischemia. Recovery group inhaled 1.4% isoflurane 30min immediately after reperfusion. Collect sober, ischemia and reperfusion microdialysis specimens, determination of glutamate neurotransmitter concentration. And double-blind motor function assessment. Double-blind counting hippocampal CA1 core complete pyramidal cells and apoptotic cells percentage. Results Compared with the control group, the concentration of glutamate neurotransmitter in the hippocampus of the resuscitation group was lower than that of the control group (P <0.05), while the motor function score of the resuscitation group increased 15 min after ischemia (P <0.05) The apoptotic rates of hippocampus neurons in control group and resuscitation group at 10, 15, 20min ischemia were lower than those in sham operation group (P <0.05) <0.05). Conclusion Inhalation of isoflurane in the early stage of reperfusion has a protective effect on brain. Its mechanism is related to the promotion of glutamate neurotransmitter during cerebral ischemia reperfusion period.