论文部分内容阅读
目的探讨1980-2015年从东南沿海地区不同地域、时间、宿主、媒介及患者中分离的汉坦病毒(HV)的遗传进化及流行规律。方法运用遗传流行病学、分子流行病学和生物信息学方法分析1980-2015年东南沿海地区(江苏、浙江、福建省和上海市)HV的S、M片段高变区基因的变异位点与频率,结合HV、宿主、媒介与生态环境4个方面及流行病学资料,分析HV在不同地域、时间、自然宿主以及重要传播媒介之间的演变规律。结果东南沿海地区肾综合征出血热(HFRS)临床患者感染的病毒以汉滩型(HTN)HV居多,主要与76-118株同源为主,汉城型病毒(SEOV)与Z37株相似的病毒为主;为HV HTN和SEO的混合型疫区,呈现高度的地理聚集现象;鼠类携带HV以SEOV为主,病毒分支与携带宿主的种类和采集地点有关,SEOV主要来源于褐家鼠,HTNV主要来源于黑线姬鼠。结论 HV和HFRS疫区的形成和维持具有一定的内在规律。
Objective To investigate the genetic evolution and prevalence of Hantaviruses (HV) isolated from different regions, time, hosts, vectors and patients in southeastern coastal areas from 1980 to 2015. Methods The genetic variations of the HV and S region of HV in the southeastern coastal areas (Jiangsu, Zhejiang, Fujian and Shanghai) from 1980 to 2015 were analyzed using genetic epidemiology, molecular epidemiology and bioinformatics methods. Frequency, combined HV, host, media and ecological environment and epidemiological data in four areas, HV in different regions, time, natural host and the evolution of important media between. Results The majority of Hantaan type (HTN) HVs were found in clinical samples of patients with hemorrhagic fever with renal syndrome (HFRS) in the southeastern coastal areas, which were predominantly homologous with 76-118 strains. The virus of SEOV-type was similar to that of Z37 strain Mainly HV HVNN and SEO hybrid epidemic areas, showing a high degree of geographical aggregation; HV carrying HV SEOV-based virus, the branch of the virus carrying host species and collection sites, SEOV mainly from Rattus norvegicus, HTNV mainly comes from Apodemus agrarius. Conclusion The formation and maintenance of HV and HFRS epidemic areas have some inherent laws.