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通过部分酸水解和甲基化分析,结合GC-MS测试手段,对当归多糖ASP3的糖链结构进行了研究.采用0.2mol/L三氟乙酸(Trifluoroacetic acid,TFA)对ASP3进行了部分酸水解,对水解前后的多糖组分进行甲基化分析.结果显示:ASP3的糖残基主要由D-GalpA,D-Galp,L-Araf和L-Rhap组成,主链由1,4-D-GalpA连接,形成“光滑区”半乳糖醛酸聚糖,由1,4-D-GalpA通过O-4位与1,2-;1,2,4-L-Rhap的O-2位交替连接形成含有较多分支的“毛发区”鼠李半乳糖醛酸聚糖.58.8%的Rhap残基发生O-4位取代(1,2,4-L-Rhap).由T-,1,5-,1,3,5-Araf和T-,1,3-,1,3,6-,1,4-,1,4,6-D-Galp聚合形成的阿拉伯半乳聚糖、半乳聚糖以及阿拉伯聚糖是ASP3侧链的主要组成,通过Rhap残基的O-4位与主链相连.
Through partial acid hydrolysis and methylation analysis combined with GC-MS testing methods, the sugar chain structure of Angelica polysaccharide ASP3 was studied. Partial acid hydrolysis of ASP3 was performed using 0.2 mol/L Trifluoroacetic acid (TFA). The methylation analysis was performed on the polysaccharide components before and after hydrolysis. The results showed that the sugar residues of ASP3 are mainly composed of D-GalpA, D-Galp, L-Araf, and L-Rhap, and the main chain consists of 1,4-D-. GalpA linkage, forming a “smooth zone” of galacturonic acid glycans, from 1,4-D-GalpA through the O-4 position to 1,2-; 1,2,4-L-Rhap O-2 Alternately linking Rhap residues with 58.8% Rhap residues containing more branched “hair zone” galacturonic acid glycans, O-4-substituted (1,2,4-L-Rhap). Arabis galactomerization formed by the polymerization of 1,5-,1,3,5-Araf and T-,1,3-,1,3,6-,1,4-1,4-1,4-6-D-Galp Sugar, galactan, and arabinan are the main components of the ASP3 side chain and are linked to the main chain through the O-4 position of the Rhap residue.