论文部分内容阅读
目的分析妊高症患者及新生儿脐血血清血管内皮细胞钙黏蛋白(VE-cadherin)和高迁移率蛋白-1(HMGB1)的变化。方法采用酶联免疫吸附法(ELISA)测定98例妊高症患者(包括妊娠期高血压40例、轻度子痫前期30例和重度子痫前期28例)及30例正常对照组产妇及新生儿脐血血清VE-cadherin和HMGB1含量。结果VE-cad herin和HMGB1在妊高症组及正常对照组及新生儿脐血血清中均有表达,VE-cadherin在三组妊高症病患者血清中含量与正常对照组比较,差异有显著意义(t=5.83,8.41,9.04,P均<0.05)。随着病情越严重,VE-cadherin水平越高,三组之间差异有统计学意义(t=6.23,8.56,P均<0.05);HMGB1与VE-cadherin分布高度一致,并呈正相关(r=0.59,P<0.01)。VE-cadherin和HMGB1在妊高症各组新生儿脐血中的含量,差异无统计学意义(P>0.05)。结论VE-cadherin和HMGB1的高表达,显示妊高症病患者内皮细胞损伤,是妊高症内皮损伤炎症标记物,为妊高症的治疗和预防开辟一条新途径
Objective To analyze the changes of VE-cadherin and HMGB1 in umbilical blood serum of hypertensive patients and neonates. Methods 98 cases of pregnancy induced hypertension (including 40 cases of gestational hypertension, 30 cases of mild preeclampsia and 28 cases of severe preeclampsia) and 30 normal controls were measured by enzyme-linked immunosorbent assay (ELISA) Cord blood serum levels of VE-cadherin and HMGB1. Results The expression of VE-cadherin and HMGB1 in the hypertensive group, the normal control group and neonatal umbilical serum were significantly higher than those in the normal control group Significance (t = 5.83,8.41,9.04, P <0.05). The more severe the disease, the higher the level of VE-cadherin, the difference between the three groups was statistically significant (t = 6.23, 8.56, P <0.05). The distribution of HMGB1 and VE-cadherin was highly consistent and positively correlated 0.59, P <0.01). The levels of VE-cadherin and HMGB1 in umbilical blood of newborn infants with pregnancy-induced hypertension were not significantly different (P> 0.05). Conclusions The high expression of VE-cadherin and HMGB1 shows endothelial cell injury in PIH patients and is a marker of inflammation in PIH, which opens up a new way for the treatment and prevention of PIH.