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目的 研究P2 1和P15蛋白在慢性肝炎、原发性肝癌肝组织中的表达及与肝细胞凋亡的关系。方法 对 36例慢性肝炎、10例原发性肝癌肝组织进行P2 1和P15蛋白免疫组化及肝组织DNA损伤检测。结果 P2 1和P15蛋白在慢性病毒性肝炎 (CH)肝组织中的表达主要为肝细胞核阳性 ,而原发性肝癌则均表达在癌细胞的胞质中 ,在细胞核中无表达 ;中重度CH(G3～G4)患者P2 1、P15蛋白的表达水平显著高于轻度 (G1～G2 )患者 ,与CH肝组织DNA损伤的相关系数分别为 0 5 6、0 5 1(P <0 0 1)。结论 P2 1和P15蛋白参与了CH肝组织的病理损伤及肝细胞凋亡。
Objective To study the expression of P2 1 and P15 proteins in chronic hepatitis and primary liver cancer and their relationship with hepatocellular apoptosis. Methods Immunohistochemical staining of P2 1 and P15 proteins and DNA damage in liver tissue were performed in 36 patients with chronic hepatitis and 10 with primary liver cancer. Results The expression of P2 1 and P15 proteins in liver tissues of chronic viral hepatitis (CH) was mainly positive for hepatocytes, while that of primary liver cancer was all expressed in the cytoplasm of cancer cells without expression in the nucleus. The expression levels of P2 1 and P15 in G3 ~ G4 patients were significantly higher than those in patients with mild G1 ~ G2, and the correlation coefficients with DNA damage in CH patients were 0 56.0 51 (P 0 01) . Conclusion P2 1 and P15 proteins are involved in the pathological damage and hepatocellular apoptosis of CH liver.