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目的:研究黄连吴茱萸不同配比在Caco-2单层细胞模型中的跨膜转运,比较小檗碱等四种主要化学成分表观穿透系数Papp的异同。方法:复制Caco-2细胞模型,采用HPLC测定转运后药液5种化学成分含量,并计算吸收系数,考察黄连吴茱萸不同配比时小檗碱等四种化学成分在细胞模型中的吸收特征。结果:随着配比中黄连比例的增加,无论是AP-BL还是BL-AP,4种化学成分的Papp均随时间的推移呈线性增加趋势,即黄连:吴茱萸为6:1时吸收率最大,差异有显著性(P<0.01)。结论:增加黄连的配伍比例,有利于黄连和吴茱萸主要化学成份的吸收,黄连吴茱萸为6:1时各主要化学成分的吸收最多。
OBJECTIVE: To study the transmembrane transport of Coptis chinensis with different ratios in the Caco-2 monolayer cell model, and to compare the similarities and differences of the apparent permeability coefficient Papp between the four major chemical components such as berberine. METHODS: The Caco-2 cell model was replicated, and the contents of five chemical components in the post-transport drug solution were determined by HPLC, and the absorption coefficients were calculated. The absorption characteristics of four chemical components such as berberine in different proportions of Coptis chinensis and E. urophylla were investigated. Results: With the increase of the ratio of Rhizoma Coptidis in the combination, whether the AP-BL or BL-AP, the Papp of the four chemical components increased linearly with time, that is, the maximum absorption rate of Coptis chinensis was 6:1. There was a significant difference (P<0.01). Conclusion: The increase of the proportion of Coptidis Rhizoma is beneficial to the absorption of major chemical constituents of Coptis chinensis and E. variegata, and the absorption of major chemical constituents is the most when Coptis chinensis is 6:1.