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Objective To explore the role and significance of matrix metalloproteinase-9(MMP-9)in angiogenesis through observing the relationship between the expression of MMP-9 and microvessel density(MVD)in glioma.Methods The expressions of MMP-9 and CD34 in 10 cases of normal brain tissues and 58 cases of glioma(14 cases of grade Ⅰ,20 cases of grade Ⅱ,15 cases of grade Ⅲ,and 9 cases of grade Ⅳ)were detected by immunohistochemical streptavidin-peroxidase technique.The positive cells of MMP-9 and the positive microvessels were examined under binocular light microscope.Results The positive expression of MMP-9 in glioma was located in the tumor-cell cytoplast and endothelial cells.The positive rate of MMP-9 in glioma of grade I,II,III and IV was 42.9%,65.0%,86.7% and 88.9%,respectively.The expression of MMP-9 was obviously higher than that of normal brain tissues(P<0.01)and positively correlated with glioma malignancy(rs=0.597,P<0.05).MVD was correlated with glioma malignancy(H=47.865,P<0.05).The expression of MMP-9 was significantly correlated with MVD(rs =0.897,P<0.01).Conclusion The expressions of MMP-9 and MVD are correlated with glioma malignancy,which may be helpful in judging the malignancy,invasion and prognosis.MMP-9 plays an important role in angiogenesis of glioma and accelerates glioma malignancy development by promoting angiogenesis.
Objective To explore the role and significance of matrix metalloproteinase-9 (MMP-9) in angiogenesis through observing the relationship between the expression of MMP-9 and microvessel density (MVD) in glioma. Methods The expressions of MMP-9 and CD34 in 10 cases of normal brain tissues and 58 cases of glioma (14 cases of grade Ⅰ, 20 cases of grade Ⅱ, 15 cases of grade Ⅲ, and 9 cases of grade Ⅳ) were detected by immunohistochemical streptavidin-peroxidase technique. The positive cells of MMP -9 and the positive microvessels were examined under binocular light microscope. Results of the positive expression of MMP-9 in glioma was located in the tumor-cell cytoplast and endothelial cells. The positive rate of MMP-9 in glioma of grade I, II, III and IV were 42.9%, 65.0%, 86.7% and 88.9%, respectively. The expression of MMP-9 was obviously higher than that of normal brain tissues (P <0.01) and positively correlated with glioma malignancy <0.05) .MVD was correlated with glioma malignancy (H = 47.865, P <0.0 5) .The expression of MMP-9 was significantly correlated with MVD (rs = 0.897, P <0.01) .Conclusion The expressions of MMP-9 and MVD are correlated with glioma malignancy, which may be helpful in judging the malignancy, invasion and prognosis. MMP-9 plays an important role in angiogenesis of glioma and accelerates glioma malignancy development by promoting angiogenesis.