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利用“鲁保一号菌”(Colletotrichum gloeos porioides(Penz)Sace.f.sp.Cuscutae)生物防除为害大豆、胡麻的菟丝子,效果显著,曾在全国20多个省(区)推广应用。但是,由于该菌的菌种在人工培养过程中,极易发生形态的变异和致病力的退化而丧失实用价值,直接影响到鲁保一号菌剂的生产和应用效果,使我国目前仅有的这项利用微生物防除杂草技术的发展,受到严重威协。中国农业科学院土肥所微生物研究室,1982~1987年开展了这方面的研究,并得到农牧渔业部科技司的资助。通过5年工作,对该菌的变异现象,做了长期系统的观察和研究;并从原始菌株中,分离到培养特征和致病力等方面,显著不同的4个单孢变异类型;找出了菌种形态特征与致病力的相关议;选育出一株经过连续人工传代培养150代,
The use of "Colletotrichum gloeos porioides (Penz) Sace.f.sp.Cuscutae biological control of soybean, flaxseed dodder, a significant effect, has been in more than 20 provinces (regions) to promote the use of. However, due to the strain of bacteria in the process of artificial cultivation, prone to morphological variation and the loss of pathogenicity and loss of practical value, a direct impact on the production and application of the effect of a strain of Lupu First, so far only Some of this use of micro-organisms to control weeds technology development, have been severely weakened. Soil and Fertilizer Institute of Chinese Academy of Agricultural Sciences, Department of Microbiology, from 1982 to 1987 to carry out research in this area, and the Ministry of Agriculture, Animal Husbandry and Fisheries Department of Science and Technology funding. After 5 years of work, the phenomenon of variation of this strain was observed and studied in a long-term system. From the original strains, 4 different types of single spore mutation were found, which were significantly different in culture characteristics and pathogenicity. The strain morphological characteristics and pathogenicity of the relevant protocols; breeding a continuous artificial subculture of 150 generations,