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为研究水稻赤霉素20-氧化酶2(OsGA20ox2)基因的过量表达对水稻株高和生长发育的影响,通过RT-PCR扩增OsGA20ox2基因并构建该基因的过量表达载体pS12Q,采用农杆菌介导法遗传转化水稻,获得了高秆转基因植株。获得T0代的85株中,对其叶片基因组DNA进行Hyg基因扩增,发现其中2株为转基因植株且表现为高秆。分析其T2代表型,高秆植株与对照相比,株高增加9.41%(P<0.05);穗颈长增加15.62%(P<0.05);倒1节间长度增加8.46%(P<0.05);穗长增加7.98%(P<0.05);穗粒数增加6.8%(P<0.05);分蘖数增加了12.5%(P<0.05);百粒重增加2.6%(P<0.05);开花提前10天。这些性状导致高秆植株的产量增加,生育周期缩短。RT-PCR结果显示OsGA20ox2基因在高秆植株茎中的表达高于对照,促进了水稻茎的延伸。结果表明OsGA20ox2基因的过量表达影响了水稻的株高和生长发育。
In order to study the effect of overexpression of gibberellin 20-oxidase 2 (OsGA20ox2) on the plant height and growth of rice, the OsGA20ox2 gene was amplified by RT-PCR and the over-expression vector pS12Q was constructed. Agrobacterium-mediated Guidance method of genetic transformation of rice, access to high stalks transgenic plants. Among 85 strains of T0 generation, Hyg gene amplification was carried out on the genomic DNA of leaves. Two of them were transgenic plants and showed high stalks. Compared with the control, the plant height increased 9.41% (P <0.05), the neck length increased 15.62% (P <0.05), and the length of the internode increased by 8.46% (P <0.05) ; Spike length increased by 7.98% (P <0.05); grain number per spike increased by 6.8% (P <0.05); tiller number increased by 12.5% (P <0.05) 10 days. These traits lead to increased production of tall stalks and shorter growth cycles. The result of RT-PCR showed that the expression of OsGA20ox2 gene in stems of high-stalk plants was higher than that of the control, which promoted the elongation of rice stems. The results showed that overexpression of OsGA20ox2 gene impacted plant height and growth of rice.