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目的对2014年安徽省一起人感染布鲁菌疑似患者进行表型和分子生物学检测,以明确诊断。方法对患者进行流行病学调查,采用血培养法对经试管凝集试验检测为布鲁菌抗体阳性的患者和病羊血液进行荧光PCR及细菌分离培养,应用传统方法和分子生物学方法对可疑菌落进行种型鉴定。结果 136份高危者及疑似布病患者血清抗体筛查22份阳性,31份患者、病羊和外环境采样标本经荧光PCR检测5份阳性。2株安徽省分离株经传统方法和种型特异性PCR均鉴定为羊种生物3型布鲁菌。结论从患者和病羊均分离出羊种生物3型布鲁菌,结合流行病学资料分析,安徽省此次疫情由人直接接触病羊而感染布鲁菌所致。
Objective To investigate the phenotype and molecular biology of a human suspected brucellosis in Anhui Province in 2014 to confirm the diagnosis. Methods The epidemiological investigation of the patients was carried out. Fluorescent PCR and bacterial isolation were performed on the blood of patients with positive brucella antibody detected by the test tube agglutination test and by blood culture method. The suspicious bacterial colonies were detected by the traditional methods and molecular biology methods For species identification. Results Totally 22 positive and 31 patients were screened for serum antibodies in 136 high-risk and suspected brucellosis patients. Five positive samples were detected by fluorescence PCR in diseased sheep and external environment samples. Two strains of Anhui isolates were identified as Brucella 3 Brucella by traditional methods and type-specific PCR. Conclusion Brucella 3 was isolated from both patients and diseased sheep. According to the epidemiological data, brucellosis was caused by direct contact with sick sheep in Anhui Province.