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为研究凡隆气单胞菌在人工感染斑点叉尾鮰体内的动态分布规律及其致病机理,用凡隆气单胞菌(8×107 CFU/mL)分别以浸泡和腹腔注射的方式感染斑点叉尾鮰,于接种后第1、2、6、12、24、48、72和96小时剖杀采样,制备石蜡切片,HE染色,镜检,并采用亲和免疫组织化学方法检测凡隆气单胞菌在斑点叉尾鮰体内的动态分布。结果显示,浸泡组在接种后第2小时,首先在鳃检测到阳性信号,6h后从脾和肾中检测到阳性信号,随后在肝和脑中也有少量阳性信号被检出;腹腔注射组在接种后第2小时即从脾和肾中检测到阳性信号,随后肝中也检测到阳性信号,而鳃中没有检测到阳性信号。两种感染方式阳性信号在各组织器官内的分布相似,主要出现在肾、脾和肝的吞噬细胞内、血管内、坏死区域、细胞间质区域以及少量实质细胞内,引起多器官的充血、出血、全身性炎症及变性和坏死,并随着感染时间的延长,检出量逐渐增多,肌肉、心脏、肠和眼球组织中没有检测到阳性信号。结果表明,凡隆气单胞菌感染途径多样化,自然环境中可通过鳃进入斑点叉尾鮰机体,肝、肾、脾是主要的感染靶器官,靶器官的损伤是造成斑点叉尾鮰死亡的重要原因之一。
In order to investigate the dynamic distribution and pathogenesis of Aeromonas hydrophila in artificial infection of Ictalurus punctatus, the infection of Ictalurus punctatus by immersion and intraperitoneal injection with Aeromonas hydrophila (8 × 107 CFU / mL) The samples were harvested at 1, 2, 6, 12, 24, 48, 72 and 96 hours after inoculation. Paraffin sections were prepared, stained with HE, examined microscopically and detected by immunohistochemistry. Dynamic distribution in channel catfish. The results showed that at 2 hours after inoculation, the immersion group first detected a positive signal in the gill and positive signals in the spleen and kidney 6 hours later, followed by a small amount of positive signals in the liver and brain. The intraperitoneal injection group Positive signals were detected in the spleen and kidney at 2 hours after inoculation, followed by positive signals in the liver, whereas no positive signals were detected in the gills. The positive signals of two infection patterns were similar in all tissues and organs, mainly in the phagocytic cells of kidney, spleen and liver, intravascular, necrotic areas, interstitial cells and a small amount of parenchymal cells, causing multiple organ hyperemia, Hemorrhage, systemic inflammation and degeneration and necrosis. With the prolongation of infection time, the detection quantity gradually increased, and no positive signal was detected in muscle, heart, intestine and eyeball. The results showed that all kinds of infection of Aeromonas sobria were diversified and could enter the body of channel catfish through the gills in the natural environment. The liver, kidney and spleen were the main target organs of infection. The damage of target organs was the important cause of the death of the channel catfish one.