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目的探讨蛋白酶激活受体2(PAR-2)在“肺合大肠”生理机制中的作用。方法将30只SD大鼠随机分为空白对照组、肺生理性激活组、结肠生理性激活组,每组10只。肺生理性激活组采用雾化吸入1%PAR-2激活剂反肉桂酰-亮-异亮-甘-精-亮-鸟-[酰胺](tc-LIGRLO)溶液建立肺生理性激活大鼠模型;结肠生理性激活组采用结肠内灌入1%tc-LIGRLO溶液建立结肠生理性激活大鼠模型。观察各组大鼠肺与结肠功能、肺与结肠迷走神经放电活动、支气管与结肠平滑肌张力及收缩频率。结果肺生理性激活组碳末推进率、结肠平滑肌张力及收缩频率较空白对照组明显增高,激活后10 min时迷走神经放电活动较激活前降低(P<0.05)。结肠生理性激活组气道阻力和肺动态顺应性较空白对照组明显改善,支气管平滑肌张力及收缩频率明显下降,激活后10、15 min迷走神经放电较激活前升高(P<0.05)。结论通过刺激支气管或结肠PAR-2,可使相合脏腑的功能发生改善,说明PAR-2可能在“肺合大肠”生理机制中起着维持肺与结肠气机通降功能的作用。
Objective To investigate the role of protease-activated receptor 2 (PAR-2) in the physiological mechanism of pulmonary tuberculosis. Methods Thirty Sprague-Dawley rats were randomly divided into blank control group, physiologically active lung group and physiologically active group of colon, 10 rats in each group. The lung-physiologically activated group established a rat model of lung physiology-activated activation by inhalation of 1% PAR-2 activator cinnamoyl-l-isoglucan-gan-lean-bird- [amide] The colon physiological activation group was established by intracolonic infusion of 1% tc-LIGRLO solution to establish a rat model of physiological physiological activation. Lung and colon function, lung and colon vagal discharge activity, bronchial and colonic muscle tone and contractile frequency were observed in each group. Results Compared with the blank control group, the pulmonary motility activation group showed a marked increase in the rate of carbon endoplasmic reticulum, the tension and contractility of colon smooth muscle. Compared with the control group, the activity of vagal nerve discharge decreased at 10 min after activation (P <0.05). Colonic physiological activation of airway resistance and pulmonary dynamic compliance than the blank control group was significantly improved, bronchial smooth muscle tension and contraction frequency decreased significantly, 10,15 min after activation vagus nerve discharge than before activation (P <0.05). Conclusions The function of PAR and PAR-2 may be improved by stimulating the bronchial or colon PAR-2, indicating that PAR-2 may play a role in maintaining the lung-colon colonization function in the “pulmonary colorectal” physiological mechanism.