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目的探讨骨髓间充质干细胞(BMSCs)对过氧化氢(H2O2)诱导胰岛β细胞凋亡的影响。方法以H2O2作用于大鼠胰岛瘤细胞INS-1细胞构建凋亡模型,通过Transwell装置实现INS-1细胞与BMSCs共培养。实验分为4组:单独INS-1细胞培养组(A组);INS-1+H2O2处理组(B组);BMSC+INS-1Transwell共培养组(C组);BMSC+INS-1+H2O2Transwell共培养组(D组)。MTT法检测细胞存活率,Annexin-V/PI染色流式细胞技术检测细胞凋亡水平。结果 H2O2显著降低INS-1细胞存活率,引起INS-1细胞凋亡,且呈剂量依赖性,通过Tran-swell与BMSCs共培养后,由H2O2诱导的INS-1凋亡细胞比例下降,与单独H2O2刺激组相比,细胞凋亡率下降了约26.3%,差异有统计学意义(P<0.05)。结论 BMSCs通过Transwell共培养显著抑制H2O2诱导的INS-1细胞凋亡,其作用机制可能与BMSCs旁分泌作用有关,为进一步利用BMSCs防治糖尿病提供了理论依据。
Objective To investigate the effect of bone marrow mesenchymal stem cells (BMSCs) on the apoptosis of pancreatic β cells induced by hydrogen peroxide (H2O2). Methods The apoptotic model of INS-1 cells was established with H2O2. INS-1 cells and BMSCs were co-cultured with Transwell apparatus. The experiment was divided into 4 groups: INS-1 cell culture group alone (group A), INS-1 + H2O2 treatment group (group B), BMSC + INS- Co-culture group (group D). Cell viability was detected by MTT assay and apoptosis was detected by Annexin-V / PI staining. Results H2O2 significantly decreased the survival rate of INS-1 cells and induced the apoptosis of INS-1 cells in a dose-dependent manner. After co-cultured with Tran-swell and BMSCs, the proportion of apoptotic cells induced by H2O2 decreased, Compared with the H2O2-stimulated group, the apoptotic rate decreased by about 26.3%, the difference was statistically significant (P <0.05). Conclusion BMSCs co-cultured with Transwell significantly inhibit the apoptosis of INS-1 cells induced by H2O2. The mechanism may be related to the paracrine effect of BMSCs, which provides a theoretical basis for the further use of BMSCs in the prevention and treatment of diabetes mellitus.