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目的 了解慢性粒细胞白血病 (CML)患者CD3+ 、CD4 + 和CD8+ 细胞中T细胞受体重排删除DNA环 (TRECs)的含量 ,从而推测CML患者的胸腺近期输出功能。方法 利用实时定量PCR(Taq Man)方法检测 8例CML患者外周血单个核细胞、3例CML患者CD4 + 和CD8+ 细胞中TRECs的水平。并根据外周血中CD3阳性率计算CD3+ 细胞中TRECs水平。 9例正常人外周血作为对照。结果 CML患者中TRECs含量为 0 .1± 0 .2 2 / 10 0 0T细胞 ,明显低于正常人TRECs水平 (6 .84± 4 .71/ 10 0 0T细胞 ,P <0 .0 1)。其中 5例患者外周血单个核细胞中未能检测到TRECs ,经分选CD4 + 和CD8+ 细胞后 ,2例可以检测到低水平的TRECs。结论 本研究首先报道了CML患者TRECs水平情况 ,结果显示其胸腺近期输出功能明显降低
Objective To investigate the content of T cell receptor rearrangement deletion DNA loops (TRECs) in CD3 +, CD4 + and CD8 + cells in patients with chronic myeloid leukemia (CML), and to estimate the recent thymic output function in CML patients. Methods The levels of TRECs in CD4 + and CD8 + cells from peripheral blood mononuclear cells of 8 CML patients and 3 CML patients were detected by real-time quantitative polymerase chain reaction (TaqMan). The level of TRECs in CD3 + cells was calculated according to the positive rate of CD3 in peripheral blood. Nine cases of normal peripheral blood as a control. Results The level of TRECs in CML patients was significantly lower than that of normal controls (0.84 ± 4.71 / 100 T cells, P <0.01). TRECs were undetectable in peripheral blood mononuclear cells of 5 patients. After sorting CD4 + and CD8 + cells, 2 cases could detect low levels of TRECs. Conclusion This study first reported the level of TRECs in CML patients, the results showed that the short-term output of thymus was significantly reduced