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目的观察八肽胆囊收缩素(CCK-8)对大鼠糖尿病性白内障(DC)的影响。方法用链脲霉素(STZ)复制大鼠DC模型。实验分为对照组、STZ组和治疗组。分别于实验第20、40和60天时检测晶状体iNOS mRNA与蛋白表达,一氧化氮(NO)含量与一氧化氮合酶(NOS)活性变化及组织学改变。结果对照组晶状体iNOS mRNA与蛋白未见明显表达,NO含量较少,NOS活性较低,晶状体上皮细胞(LEC)形态正常;STZ组iNOS mRNA和蛋白表达明显上调,NO生成增加,NOS活性增强,LEC病变随时间延长而加重。治疗组上述改变明显减轻。结论CCK-8可减轻晶状体损伤,机制可能与抑制iNOS基因表达、减少NO生成有关。
Objective To observe the effect of octapeptide cholecystokinin (CCK-8) on diabetic cataract (DC) in rats. Methods Streptozotocin (STZ) was used to replicate rat DC model. The experiment was divided into control group, STZ group and treatment group. The changes of iNOS mRNA and protein expression, nitric oxide (NO) content, nitric oxide synthase (NOS) activity and histological changes were detected on the 20th, 40th and 60th day after the experiment. Results The iNOS mRNA and protein in the control group were not obviously expressed, the content of NO was lower, the activity of NOS was lower and the morphology of lens epithelial cells (LECs) were normal. The expression of iNOS mRNA and protein in STZ group was significantly increased, NO production and NOS activity were enhanced, LEC lesions aggravate with time. The treatment group significantly reduced the above changes. Conclusion CCK-8 can reduce the lens damage, the mechanism may be related to inhibiting the expression of iNOS gene and decreasing the production of NO.