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采用双链RNA(double-stranded RNA,dsRNA)技术和非序列依赖PCR扩增(sequence-independent amplification,SIA)方法对感病地黄进行分子鉴定,并测定油菜花叶病毒(Youcai mosaic virus,YoMV)山西地黄分离物(YoMV-SX)的基因组全序列。序列测定及分析发现侵染地黄的病毒为油菜花叶病毒(Youcai mosaic virus,YoMV)。获得YoMV-SX(Gen Bank登录号JX422022)全长为6 304 nt,5’UTR长度为68 nt,3’UTR长度为236 nt,含有4个开放阅读框(open reading frame,ORF)。全序列核苷酸一致性分析显示Yo M V-SX与Tobamovirus亚组Ⅲ中分离物的一致性为90.7%~96.0%,与同属亚组Ⅰ和Ⅱ的一致性仅为50.2%~63.3%。全序列系统进化分析表明,YoMV-SX与YoMV-Wh形成一个独立分支,亲缘关系最近。这是YoMV侵染地黄的首次报道。
The molecular identification of Rehmannia glutinosa Rehmanniae by double-stranded RNA (dsRNA) and sequence-independent amplification (SIA) methods were performed. The expression of Youcai mosaic virus (YoMV) Genomic complete sequence of Shanxi Dihuang isolate (YoMV-SX). Sequence analysis and analysis revealed that the virus that infected Rehmannia glutinosa was Youcai mosaic virus (YoMV). The full length of YoMV-SX (Gen Bank accession number JX422022) was 6 304 nt, the length of 5’UTR was 68 nt, and the length of 3’UTR was 236 nt. It contained 4 open reading frames (ORFs). Nucleotide sequence analysis showed that the identity of Yo M V-SX to Tobamovirus subgroup Ⅲ was 90.7% -96.0%, which was only 50.2% -63.3% identical to that of subgroups Ⅰ and Ⅱ. Full sequence phylogenetic analysis showed that YoMV-SX formed an independent branch with YoMV-Wh, which has the closest genetic relationship. This is the first report that YoMV infected Rehmannia.