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本文运用随机扩增多态性DNA(RAPD)标记方法,从DMA水平对小麦异交群体4个子群体的遗传结构进行了研究.10个引物共检测65个位点,研究结果表明:(1)各子群体均保持了丰富的遗传变异;(2)连续五次选择对千粒重选重子群体的遗传结构影响不大;而在株高选低子群体中,随着选择次数的增加,群体的遗传变异有逐渐减小的趋势.(3)子群体间已出现一定分化但大部分遗传变异仍存在于子群体内部;其中,不同选择处理群体间的遗传距离较大,已发生了比较明显的分化;同一选择处理群体,选择次数越多,群体分化程度越大,另外,在本研究数据处理中,首次将RAPD谱带结果转化为基因频率分析群体遗传结构,得到与其它分析方法相吻合的结论
In this paper, the random amplified polymorphic DNA (RAPD) marker method was used to study the genetic structure of 4 outgroups of wheat outcrossing population from DMA level. A total of 65 loci were detected by 10 primers. The results showed that: (1) Genetic variation was maintained in all sub-populations; (5) Sequential five selections had little effect on the genetic structure of 1000-seed weight subgroups; In the high selection low subgroup, with the increase of the selection frequency, the genetic variation of the population tended to decrease gradually. (3) Some sub-populations have been differentiated, but most of the genetic variation still exists in the sub-population. Among them, the genetic distance between the different selection and treatment groups is relatively large, and obvious differentiation has occurred. In the same selection and treatment groups, the number of selection In the data processing of this study, the results of the RAPD bands were first transformed into the genetic structure of genetic frequency analysis population, and the conclusion is consistent with other analysis methods