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目的 :研究 p5 3、c myc、nm2 3癌基因蛋白的表达与喉癌复发的关系 ,对预后作出估计。 方法 :回顾分析喉癌手术标本 5 74例 ,术后随访 3年 ,确诊术后复发 5 6例。正常喉对照标本 3 9例。标本制成组织芯片 ,采用免疫组化SABC法对芯片标本进行染色。结果 :p5 3、nm2 3、c myc在喉癌组织中有表达 ,其阳性率各有不同。其中c myc在喉癌复发组的表达明显增强 ,与未复发组的表达差异有极显著性意义 (P <0 .0 1) ;而nm2 3在喉癌复发组的表达明显减弱 ,与未复发组的表达差异有极显著性意义 (P <0 .0 1)。p5 3在喉癌复发组与未复发组的表达差异无显著性意义 (P >0 .0 5 )。c myc的表达与喉癌复发呈正相关 ,nm2 3癌基因蛋白的表达与喉癌复发呈负相关。结论 :c myc、nm2 3癌基因蛋白的表达可作为预测喉癌术后复发的指标
Objective: To study the relationship between the expression of p53, c-myc and nm23 oncogene proteins and the recurrence of laryngeal carcinoma, and to estimate the prognosis. Methods: A retrospective analysis of 5 74 cases of laryngeal cancer specimens were followed up for 3 years after operation, and confirmed the recurrence of 56 cases. Normal laryngeal control specimens 39 cases. Specimens were made into tissue microarray and stained with SABC immunohistochemical method. Results: p5 3, nm23, c-myc were expressed in laryngeal squamous cell carcinoma with different positive rates. The expression of c-myc in laryngeal cancer recurrence group was significantly higher than that in non-relapse group (P <0.01), while the expression of nm23 in laryngeal cancer recurrence group was significantly lower than that in non-relapse group There was a significant difference in the expression of the group (P <0. 01). The expression of p5 3 in laryngeal cancer recurrence group and non-relapse group had no significant difference (P> 0.05). The expression of c-myc was positively correlated with the recurrence of laryngeal carcinoma. The expression of nm23 oncogene protein was negatively correlated with the recurrence of laryngeal carcinoma. Conclusion: The expression of c-myc and nm23 oncogene proteins can be used as an index to predict the recurrence of laryngeal cancer