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目的鉴定口服八珍汤后大鼠血中原型成分及代谢产物。方法对八珍汤给药后所得血清样品,应用HPLC-DAD-MS联用技术,在线进行选择离子扫描(SIM)、二级碎片离子全扫描,对八珍汤的体内代谢化学成分进行解析。结果大鼠口服八珍汤后从血中发现了22个入血成分,其中16个成分为八珍汤所含成分的原型,6个为新产生的代谢产物,其中原型成分分别为白术内酯Ⅰ、5-羟甲基-2-糠酸、羟基芍药苷、白术内酯Ⅲ、芍药内酯苷、芍药苷、甘草苷、阿魏酸、藁本内酯、人参皂苷Rg1、人参皂苷Rb1;代谢产物分别为芍药苷代谢素Ⅰ、3-O-β-D-18β-甘草次酸单葡萄糖醛酸苷、甘草次酸、人参皂苷Rh1、人参皂苷Rd。结论为揭示八珍汤的药效物质基础、阐明中药复方的配伍原则及质量标准制定提供科学的理论依据。
Objective To identify the prototype blood components and metabolites of rats after oral administration of Bazhen Decoction. Methods The serum samples from Bazhen decoction were analyzed by HPLC-DAD-MS combined with SIM and second-level fragment ion on-line to analyze the metabolic components of Bazhen decoction. Results After oral administration of Bazhen decoction, 22 blood components were found in the blood, of which 16 were the prototypes of Bazhen Tang and 6 were newly produced metabolites. The prototypes were atractylodes Ⅰ, 5-hydroxymethyl-2-furoic acid, hydroxy paeoniflorin, atractylenolide Ⅲ, albiflorin, paeoniflorin, liquiritin, ferulic acid, ligustilide, ginsenoside Rg1, ginsenoside Rb1; Metabolites were paeoniflorin Ⅰ, 3-O-β-D-18β-glycyrrhetinic acid monoglucuronide, glycyrrhetinic acid, ginsenoside Rh1 and ginsenoside Rd respectively. Conclusion To reveal the pharmacological basis of Bazhen Decoction and elucidate the principle of compatibility of TCM compound prescriptions and the formulation of quality standards, it provides a scientific theoretical basis.