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目的建立鹅红细胞的醛化方法,用于百日咳疫苗生产中的血凝活性测定。方法以新鲜鹅红细胞为对照,分别采用方法一、方法二醛化鹅红细胞,比较两种处理方法制备的醛化鹅红细胞的溶血性和活性,确定最佳醛化条件。结果采用方法一制备的醛化鹅红细胞的实验效果优于方法二,其测定结果与新鲜红细胞一致,在4℃保存90 d未出现溶血现象,戊二醛最佳处理浓度为1.25%,醛化时间为40 min,最佳醛化鹅红细胞工作浓度为0.8%。结论已成功建立鹅红细胞的醛化方法。
Objective To establish a method for the hydroformylation of goose erythrocytes and to determine the hemagglutination activity of pertussis vaccine. Methods Using fresh goose erythrocytes as control, the methods of first and second methods were used to dialyze the goose erythrocytes respectively. The hemolytic activity of the goblet cells was compared between the two methods to determine the optimum conditions for the hydroformylation. Results The results of albino goose red blood cells prepared by method one were better than those of method two. The results were consistent with that of fresh erythrocytes. No hemolysis occurred after 90 days of storage at 4 ℃. The optimum concentration of glutaraldehyde was 1.25% Time was 40 min, the optimal working concentration of aldehyde red goose cells was 0.8%. Conclusion The method of hydroformylation of goose erythrocytes has been successfully established.