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[Objective] This study was to reveal the effects of growth stage, age and process of refining on medicinal quality of Dipsacus asperoides. [Method] Employing high performance liquid chromatography (HPLC), the contents of saponin Ⅵ in cultivated Dipsacus asperoides of different harvest stages, different tissues and different processes of refining and the dynamic changes were determined. [Results] With saponin Ⅵ content as indicator, the optimized parameters for harvesting and processing Dipsacus asperoides materials were as follows: harvesting the three years old materials before flowering stage, drying at 105 ℃ for 30 min, then processing at 80 ℃. [Conclusion] The results provide experimental data for formulating the SOP for standardized cultivation of Dipsacus asperoides and production of medicinal industries related, and further facilitate the reasonable exploitation and sustainable use of Dipsacus asperoides resources.
[Objective] This study was to reveal the effects of growth stage, age and process of refining on medicinal quality of Dipsacus asperoides. [Method] Employing high performance liquid chromatography (HPLC), the contents of saponin VI in cultivated Dipsacus asperoides of different harvest stages, different tissues and different processes of refining and the dynamic changes were determined. [Results] With saponin Ⅵ content as indicator, the optimized parameters for harvesting and processing Dipsacus asperoides materials were as follows: harvesting the three years old materials before flowering stage, drying at 105 ° C for 30 min, then processing at 80 ° C. [Results] The results provide experimental data for formulating the SOP for standardized cultivation of Dipsacus asperoides and production of medicinal industries related, and further facilitate the reasonable exploitation and sustainable use of Dipsacus asperoides resources.