论文部分内容阅读
目的比较卒中易感型自发性高血压大鼠（ＳＨＲｓｐ）与常压（Ｗｉｓｔａｒ）大鼠基底动脉（ＢＡ）和尾动脉（ＣＡ）平滑肌收缩装置对Ｃａ２＋敏感性（以ｐＣａ５０表示）的差异。方法采用血管平滑肌细胞（ＶＳＭＣ）膜化学高通透技术并结合细胞钙钳制技术，使上述血管肌条（环）平滑肌细胞膜具有高通透性，并使胞内Ｃａ２＋固定于一定水平，然后制作神经鞘氨醇（ｓｐｈｉｎｇｏｓｉｎｅ）预温浴后的肌条（环）ｐＣａ－张力曲线，比较ＳＨＲｓｐ和Ｗｉｓｔａｒ大鼠ＢＡ和ＣＡ平滑肌在不同处理时的ｐＣａ５０值。结果不论ＳＨＲｓｐ或Ｗｉｓ－ｔａｒ大鼠，ＢＡ平滑肌收缩装置对Ｃａ２＋的敏感性均高于ＣＡ（Ｐ＜０．００５）。ＳＨＰｓｐ的ＢＡ和ＣＡ平滑肌收缩装置对Ｃａ２＋的敏感性均高于Ｗｉｓｔａｒ大鼠的相应血管（Ｐ＜０．００５）。蛋白激酶Ｃ（ＰＫＣ）抑制剂Ｓｐｈｉｎｇｏｓｉｎｅ可使ＳＨＲｓｐＣＡ平滑肌ｐＣａ－张力曲线右移，但不能使ＳＨＲｓｐＣＡ平滑肌ｐＣａ－张力曲线右移，甚至轻微左移。结论ＳＨＲｓｐＢＡ和ＣＡ平滑肌收缩装置对Ｃａ２＋的敏感性均高于Ｗｉｓｔａｒ大鼠，ＰＫＣ在这种Ｃａ２＋敏感性变化中所起的作用在ＢＡ和ＣＡ可能不同
Objective To compare the Ca2 + sensitivity (expressed as pCa50) between basilar artery (BA) and tail artery (CA) smooth muscle contracting devices in stroke-prone spontaneously hypertensive rats (SHRsp) and Wistar rats. Methods Vascular smooth muscle cells (VSMC) membrane chemical high-pass technique combined with cell calcium clamp technique was used to make the vascular smooth muscle cell membrane with high permeability and to fix intracellular Ca2 + to a certain level, then to make nerve Muscle (loop) pCa-tension curves after sphingosine pre-warming bath compared the pCa50 values of BA and CA smooth muscle of SHRsp and Wistar rats at different treatments. Results The sensitivity of BA smooth muscle contraction device to Ca2 + was higher than that of CA (P <0.005) in either SHRsp or Wis-tar rats. SHPsp BA and CA smooth muscle contraction device of Ca2 + sensitivity were higher than Wistar rats corresponding blood vessels (P <0.005). Sphingosine, a protein kinase C (PKC) inhibitor, shifted the pCa-tension curve of the SHRspCA smooth muscle to the right, but did not shift the pCa-tension curve of the SHRspCA smooth muscle to the right or even to the left. Conclusions The sensitivity of SHRspBA and CA smooth muscle contraction devices to Ca2 + is higher than that of Wistar rats. The role of PKC in this Ca2 + sensitivity change may be different between BA and CA