论文部分内容阅读
选取12株已知性别成年银杏和27株未知性别银杏幼苗为材料,将成年植株与幼苗的叶形特征及SCAR标记特异带进行协同分析,探索成、幼年银杏间性别特征在形态和分子水平上的偶联关系.结果表明:银杏雌雄株长枝叶片叶形间的稳定差异规律表现为雌株的叶形指数(叶宽/叶长)和叶裂指数(叶裂/叶长)均大于雄株;在成、幼年植株叶形分析基础上,假设了幼苗中的扇形、多裂扇形和蒲扇形叶植株为F类,可能为雌株,假设幼苗叶不规则形、楔形和翅为M类,推测其为雄株;用SCAR-GBA组引物对27株银杏一年生实生苗DNA组进行扩增,经3次重复,其中M组12株幼苗中有11株获得了与成年雄株一致的特异带,F组15株中14株没有雄性特征带.分子技术研究结果与幼苗叶形判断假设之间的吻合率达92.6%,二者之间的偶联关系密切.研究结果证明:采用叶形特征对银杏幼苗进行早期性别鉴定在生产上是可行的.
Twelve known adult Ginkgo biloba and 27 unknown Ginkgo biloba seedlings were selected as materials to analyze the leaf characteristics and SCAR marker-specific bands of adult plants and seedlings. The results showed that the sexual characteristics of young Ginkgo biloba at the morphological and molecular level The results showed that the leaf-shape stability of the long branch of Ginkgo biloba var. Gingko showed the leaf index (leaf width / leaf length) and leaf index (leaf split / leaf length) Based on the leaf shape analysis of adult and juvenile plants, it is assumed that the fan-shaped, the multi-split fan-shaped and the palm-fan shaped leaves in the seedling are F-type and may be female. Assuming that the seedling leaves are irregular, the wedge- , Which was presumed to be male. The DNA of 27 ginkgo annual seedlings was amplified by SCAR-GBA primer. After 3 replications, 11 of 12 seedlings of M group were identical with those of adult male There were no male bands in 14 of 15 strains in group F. The coincidence rate between the results of molecular techniques and the hypothesis of seedling leaf shape determination was 92.6%, and the coupling relationship between them was close.The results show that: Characteristics Early identification of ginkgo seedlings in production feasible.