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目的观察一种多功能营养复合物对四氧嘧啶诱导的糖尿病小鼠血糖及胰腺组织病理形态的影响。方法将造模成功的昆明小鼠分为模型(MOD)组,高(HC)、中(MC)、低剂量(LC)复合物组和二甲双胍(MEL)组,同时选取正常昆明小鼠分为正常对照(Con)组和高剂量复合物对照(Hcon)组。灌胃45d后检测各组体重、FPG和血糖曲线下面积(AUCg)的变化,并测定血清胰岛素水平,观察胰腺组织病理形态。结果与Con组比较,Hcon组FPG[(5.88±0.52)vs(5.94±0.21)mmol/L]、AUCg[(14.23±1.85)vs(15.35±0.26)mmol/L]和血清胰岛素水平[(15.62±0.54)vs(15.01±0.99)mU/L]差异均无统计学意义(P>0.05)。与MOD组比较,HC、MC、LC组和MEL组FPG[(20.16±1.66)vs(11.78±1.25)vs(13.00±1.89)vs(13.79±1.37)vs(12.24±1.36)mmol/L,P<0.05)]和AUCg[(44.01±1.52)vs(32.49±2.73)vs(35.62±2.09)vs(37.39±1.88)vs(33.06±1.71),P<0.05)]降低,血清胰岛素含量增加[(8.99±0.68)vs(13.37±0.88)vs(12.86±0.63)vs(11.92±0.48)vs(14.29±0.78)mU/L,P<0.05)],高剂量复合物胰岛素敏感指数提高[(-5.50±0.21)vs(-4.91±0.13),P<0.05)]。胰腺组织切片染色结果显示,高、中、低剂量复合物和二甲双胍能够修复受损的胰岛组织,并且胰岛素表达量积分光密度值与MOD组比较升高[(77460±4849)vs(200400±11790)vs(127300±11750)vs(109900±19400)vs(194300±19400),P<0.05)]。结论该复合物能降低糖尿病小鼠FPG和AUCg,增加血清胰岛素含量,提高IS,可能主要是通过促进受损的胰岛β细胞修复实现的。
Objective To observe the effect of a multifunctional nutrition complex on blood glucose and pancreatic histopathology in alloxan-induced diabetic mice. Methods Kunming mice were divided into model group, high (HC), medium (MC), low dose (LC) complex group and metformin group, and normal Kunming mice were divided into Normal control (Con) group and high dose compound control (Hcon) group. At 45 days after gavage, the changes of body weight, FPG and the area under the curve of blood glucose (AUCg) were measured. The levels of serum insulin were measured and the pathological changes of pancreas were observed. Results Compared with Con group, FPG [(5.88 ± 0.52) vs (5.94 ± 0.21) mmol / L], AUCg [(14.23 ± 1.85) vs (15.35 ± 0.26) mmol / L] and serum insulin [(15.62 ± 0.54) vs (15.01 ± 0.99) mU / L], respectively. There was no significant difference between the two groups (P> 0.05). FPG [(20.16 ± 1.66) vs (11.78 ± 1.25) vs (13.00 ± 1.89) vs (13.79 ± 1.37) vs (12.24 ± 1.36) mmol / L in HC, MC, LC and MEL groups, (P <0.05)] and AUCg [(44.01 ± 1.52) vs (32.49 ± 2.73) vs (35.62 ± 2.09) vs (37.39 ± 1.88 vs 33.06 ± 1.71, P <0.05) (P <0.05). Compared with the control group, the insulin sensitivity index of high-dose group increased by (-5.50 ± 0.68) vs (13.37 ± 0.88) vs (12.86 ± 0.63 vs 11.92 ± 0.48 vs 14.29 ± 0.78 mU / ± 0.21 vs -4.91 ± 0.13, P <0.05)]. Pancreatic tissue sections staining showed that high, medium and low dose of compound and metformin could repair damaged islet tissue, and the integrated optical density of insulin expression increased compared with that of MOD [(77460 ± 4849) vs (200400 ± 11790 ) vs (127,300 ± 1,1750) vs (99,900 ± 19400) vs (194300 ± 19400), P <0.05)]. Conclusion The compound can reduce FPG and AUCg in diabetic mice, increase serum insulin level and increase IS level, which may be mainly through the promotion of damaged islet β cell repair.