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测定免疫复合物最常用的两个方法是C_1q结合试验(C_1q—BA)和C_1q固相试验C_1q—SP),其敏感程度常受到血清中内源C_1q含量的影响。放射标记的C_1q与聚乙二醇结合,而Zubler和Tung等在C_1q试验中向血清内加EDTA,其后许多研究者都采用了他们的方法。作者就C_1q固相试验中EDTA的干扰作用及利用辣根过氧化物酶标记的蛋白A作为免疫复合物指示剂进行了研究。 C_1q按Yonemasu和stroud的方法从正常人血清(NHS)中提取,C_1q制备物保
Two of the most commonly used methods of determining immune complexes are the C 1 q binding assay (C 1q-BA) and the C 1q solid phase assay (C 1q-SP), the sensitivity of which is often influenced by the level of endogenous C 1q in serum. Radiolabeled C_1q bound polyethylene glycol, while Zubler and Tung et al. Added EDTA to the serum in the C_1q test, and many researchers followed their approach. The authors studied the interference of EDTA in the C 1q solid-phase assay and the use of horseradish peroxidase-labeled protein A as an indicator of immune complexes. C_1q was extracted from normal human serum (NHS) by the method of Yonemasu and stroud,