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本文报告用薄层层析-红外光谱法测定莪术挥发油中莪术醇的含量。以石油醚-丙酮(96:4)为展开剂,在硅胶G板上将挥发油中极性较小的干扰物质层析至高R_f值区而将莪术醇留在原点附近,然后无需标记出莪术醇的斑点,根据经实验求出的莪术醇的R_f值直接将薄层上莪术醇部分刮下洗脱,再以红外光谱中莪术醇分子中亚乙烯基的吸收峰(面外弯曲振动890 cm~(-1),11.24μm)为定量谱带,用四氯化碳为溶剂,以红外光谱法测定莪术醇的含量。由于亚乙烯基谱带的专属性较强,除干扰组分外,其他组分与莪术醇无需严格分离。本法操作简便,结果稳定,统计结果表明,8次测定的平均相对偏差小于2％。
In this paper, the content of curcumol in essential oil of Rhizoma Zedoariae was determined by TLC-IR spectrometry. Using petroleum ether-acetone (96:4) as a developer, the less polar interfering substance in the volatile oil is chromatographed on a silica gel G plate to a high R f value zone, leaving the curcumol near the origin, and then eliminating the need to label the curcumol The spot, according to experimentally determined Curcumol alcohol R_f value directly on the thin layer Curcumol alcohol part of the scraping elution, and then in the infrared spectrum of Curcumol alcohol vinylidene absorption peak (outside-out bending vibration 890 cm ~ (-1), 11.24 μm) was a quantitative band, and the content of curcumol was determined by infrared spectroscopy using carbon tetrachloride as the solvent. Due to the strong specificity of the vinylidene band, the other components, except the interference component, do not need to be strictly separated from Curcumol. The method is simple and stable, and the statistical results show that the average relative deviation of 8 measurements is less than 2%.