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目的 :通过对变应性鼻炎豚鼠的嗜碱粒细胞释放组胺能力的测定 ,从细胞水平探讨变应性鼻炎的发病机制 ,并期望为变应性鼻炎的诊断指标提供一种客观手段。 方法 :健康豚鼠随机分组后 ,建立豚鼠变应性鼻炎模型 ,刀豆球蛋白 A(Con A)作刺激物 ,应用荧光测定法 ,比较豚鼠实验组和正常组之间嗜碱粒细胞释放组胺的能力。结果 :在 3种浓度 Con A的刺激诱导下 ,变应性鼻炎组嗜碱粒细胞组胺释放率均较正常组显著增高 (P<0 .0 1)。 结论 :变应性鼻炎的豚鼠存在着嗜碱粒细胞组胺释放能力的异常 ,组胺释放能力的增强在变应性鼻炎的发病机制中可能起一定作用
OBJECTIVE: To explore the pathogenesis of allergic rhinitis at the cellular level by measuring the release of histamine by basophils in allergic rhinitis guinea pigs, and to provide an objective measure for the diagnosis of allergic rhinitis. Methods: Guinea pigs with allergic rhinitis and con A were stimulated by healthy guinea pigs randomly. Fluorescence assay was used to compare the release of histamine from basophils in experimental group and normal group Ability. Results: Under the stimulation of three concentrations of Con A, the release of basophil histamine in allergic rhinitis group was significantly higher than that in normal group (P <0.01). CONCLUSIONS: Allergic rhinitis guinea pigs have abnormalities of basophil histamine release, and histamine release may play an important role in the pathogenesis of allergic rhinitis