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投放不同浓度梯度的枯草芽孢杆菌于凡纳滨对虾幼体养殖环境中,监测不同枯草芽孢杆菌使用浓度下与养殖环境相关的水质因子(氨氮、亚硝酸盐氮、化学需氧量)的变化;观察不同枯草芽孢杆菌使用浓度下凡纳滨对虾幼体对人工制造的胁迫环境的抗逆性;观测不同枯草芽孢杆菌使用浓度下凡纳滨对虾幼体的成活率与体重增长率。研究枯草芽孢杆菌对水质改善和凡纳滨对虾幼体抗逆性的影响。结果表明,枯草芽孢杆菌能显著(P<0.05)降低化学需氧量、氨氮含量,抑制亚硝酸盐氮的产生;当枯草芽孢杆菌投放浓度为1.25×10~4cfu/ml时,水体中化学需氧量、氨态氮、亚硝酸盐氮含量均值比对照组分别降低了65.30%、59.70%、88.64%,成活率比对照组提高了10.00%,体重增长率是对照组的2.44倍;当枯草芽孢杆菌使用浓度为1.25×10~4～1.25×10~6cfu/ml时,对虾抗逆性显著(P<0.05)增强,对虾在氨氮、亚硝酸盐氮、高锰酸钾、甲醛胁迫下24小时的成活率均值分别比对照组提高了16.94%、24.44%、44.17%、18.61%。
The Bacillus subtilis with different concentration gradients were put in the environment of Litopenaeus vannamei larval culture to monitor the changes of aquaculture factors (ammonia nitrogen, nitrite nitrogen and chemical oxygen demand) under the different concentrations of Bacillus subtilis. Under different concentrations of Bacillus subtilis Litopenaeus vannamei larvae artificially induced stress environment resistance; observed Bacillus subtilis use concentration of Litopenaeus vannamei larvae survival rate and weight gain rate. The effect of Bacillus subtilis on water quality improvement and resistance of Litopenaeus vannamei larvae was studied. The results showed that Bacillus subtilis significantly (P <0.05) decreased COD and ammonia nitrogen content and inhibited the production of nitrite nitrogen. When the Bacillus subtilis concentration was 1.25 × 10 ~ 4 cfu / ml, the chemical requirement Compared with the control group, the mean values of oxygen, ammonia nitrogen and nitrite nitrogen decreased by 65.30%, 59.70% and 88.64%, respectively, and the survival rate increased by 10.00% and 2.44 times as that of the control group respectively. When the concentration of Bacillus sp. Was 1.25 × 10 ~ 4 ~ 1.25 × 10 ~ 6cfu / ml, the resistance of shrimp was significantly (P <0.05) enhanced. Under the stress of ammonia nitrogen, nitrite nitrogen, potassium permanganate and formaldehyde The average survival rate of young children increased by 16.94%, 24.44%, 44.17% and 18.61% respectively over the control group.