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目的观察体外添加不同浓度的重组人粒细胞-巨噬细胞集落刺激因子(rhGM-CSF)对人精子运动参数的影响,探讨其在精子运动中的作用机制。方法健康生育男性和弱精子症患者各10例手淫取精,经简易上游优化处理后的精子与不同浓度rhGM-CSF溶液孵育10min、30min、60min后,采用计算机辅助的精液分析系统检测精子各项运动参数的变化。结果对精液运动参数正常的标本,1ng/ml和10ng/ml的rhGM-CSF溶液能显著提高精子的活率、前向性运动百分率,在10ng/ml时平均直线运动速度也有显著提高。对弱精子症患者精液标本,1ng/ml和10ng/ml的rhGM-CSF溶液能显著提高精子的前向运动百分率和直线运动速度,10ng/ml的rhGM-CSF对精子活率和平均路径速度也有显著性提高。两类精子的曲线运动速度均无显著性变化。结论1ng/ml～10ng/ml浓度范围的rhGM-CSF体外能显著改善精子的运动功能。
Objective To observe the effects of rhGM-CSF on the sperm motility parameters of human sperm in vitro and to explore the mechanism of rhGM-CSF on sperm motility. Methods 10 males with healthy fertility and asthenospermia were masturbated and masturbated respectively. The sperm optimized by simple upstream optimization was incubated with different concentrations of rhGM-CSF for 10 minutes, 30 minutes and 60 minutes. The computer-assisted semen analysis system was used to detect sperm Changes in exercise parameters. Results The sperm motility, forward motility and average linear motility at 1ng / ml and 10ng / ml of rhGM-CSF solution were significantly increased in sperms with normal sperm motility parameters. For sperm samples of asthenospermia patients, 1ng / ml and 10ng / ml rhGM-CSF solution can significantly increase the percentage of sperm motility and sperm motility, and 10ng / ml of rhGM-CSF on sperm motility and average path velocity Significantly improved. There was no significant change in the curve velocity of both sperms. Conclusion The concentration of rhGM-CSF in the range of 1ng / ml ~ 10ng / ml can significantly improve sperm motility.