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[目的]研究鱼藤酮引起的星形胶质细胞内游离钙浓度变化及其离子流动机制。[方法]以体外培养的大鼠中脑星形胶质细胞为研究对象,激光共聚焦结合Fluo-4荧光法动态测定细胞内游离钙浓度。[结果]鱼藤酮可浓度依赖性地引起星形胶质细胞内游离钙浓度升高。细胞内钙库释放抑制剂TMB-8、无钙缓冲液和钙通道阻滞剂MK-801、尼莫地平均可抑制鱼藤酮引起的细胞内钙浓度变化,但其抑制作用发挥的时间和程度均有显著不同。[结论]细胞内钙释放与细胞外钙内流共同参与了鱼藤酮引起的星形胶质细胞内游离钙浓度的升高,但细胞内钙释放发挥作用的时间稍迟,而鱼藤酮引起的细胞外钙内流主要由电压依赖式钙通道内流引起。
[Objective] To study the change of intracellular free calcium concentration induced by rotenone and the mechanism of its ion flux. [Method] Rat middle mesenchymal astrocytes cultured in vitro were studied by confocal laser scanning microscopy and Fluo-4 fluorescence method. [Result] Rotenone induced a concentration-dependent increase of intracellular free calcium concentration in astrocytes. Intracellular calcium release inhibitor TMB-8, calcium-free buffer and calcium channel blocker MK-801, nimodipine can inhibit the intracellular calcium concentration caused by rotenone on average, but the time and extent of its inhibitory effect There are significant differences. [Conclusion] Both intracellular calcium release and extracellular calcium influx play a role in the increase of intracellular free calcium concentration induced by rotenone. However, intracellular calcium release takes a little later, whereas rotenone induced extracellular Calcium influx is mainly caused by voltage-dependent calcium channel influx.