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以兔眼蓝莓(Vaccinium ashei)优良品种‘粉蓝’(‘Powderblue’)当年新发半木质化带腋芽茎段为外植体材料,诱导腋芽萌发得到无菌种苗。选取无菌种苗幼嫩叶片,诱导叶片直接分化产生不定芽芽丛,然后对不定芽进行壮苗培养,形成了再生植株。结果表明:最佳的丛生芽诱导培养基为WPM+0.2 mg·L~(-1)噻苯隆(TDZ)+0.1 mg·L~(-1)萘乙酸(NAA);最佳的壮苗培养基为WPM+0.5 mg·L~(-1)玉米素(ZT)+0.05 mg·L~(-1) NAA;壮苗后进行瓶内生根,最佳的生根培养基为1/2WPM+0.8 mg·L~(-1)吲哚丁酸(IBA)+0.1 g·L~(-1)活性炭(AC)。该技术体系的建立大大缩短了组培扩繁的周期。
The germinated axillary buds were induced to germinate by using axillary bud stem segments of newly developed semi-ligninized tapes of ’Vaccinium ashei’ in the same year. The young leaves of sterile seedlings were selected to induce the direct differentiation of leaves to produce adventitious buds, and then the adventitious buds were grown into strong seedlings to form regenerated plants. The results showed that the best shoot induction medium was WPM + 0.2 mg · L -1 TDZ +0.1 mg · L -1 NAA; The medium was WPM + 0.5 mg · L -1 zeatin (ZT) +0.05 mg · L -1 NAA. After rooting, the best rooting medium was 1 / 2WPM + 0.8 mg · L -1 indole butyric acid (IBA) +0.1 g · L -1 activated carbon (AC). The establishment of the technical system greatly shortened the cycle of tissue culture expansion.