论文部分内容阅读
目的:观察活血解毒方对糖尿病大鼠视网膜血管形态的影响并探讨其可能的作用机制。方法:一次性腹腔注射链脲佐菌素(Streptozotocin,STZ,65 mg/kg)诱导糖尿病大鼠模型,造模后随机分为模型组和活血解毒方组。另设正常对照组。药物干预12周后,应用视网膜消化铺片法观察视网膜血管形态;应用Western blot法检测视网膜VEGF蛋白的表达,应用Real-Time PCR法检测视网膜VEGF、Ras、Raf-1与ERK mRNA的表达。结果:模型组视网膜毛细血管面积密度及内皮细胞/周细胞比例与较正常组升高(P<0.001),VEGF蛋白及VEGF、Ras、ERKmRNA表达升高(P<0.05),Raf-1mRNA表达升高(P>0.05)。与模型组比较,活血解毒方组视网膜毛细血管面积密度和内皮细胞/周细胞比例降低(P<0.01和P<0.001),VEGF蛋白与VEGF、Ras、Raf-1、ERK mRNA表达下降(P<0.05)。结论:活血解毒方能明显抑制视网膜毛细血管和内皮细胞的增生,其机制可能是通过干预Ras/Raf-1/ERK信号转导通路,下调VEGF在视网膜中的表达,抑制血管新生,从而改善DR。
Objective: To observe the effect of Huoxue Jiedu Fang on retinal vascular morphology in diabetic rats and to explore its possible mechanism. Methods: Diabetic rats were induced by intraperitoneal injection of streptozotocin (STZ, 65 mg / kg), and were randomly divided into model group and Huoxue Jiedu Fang group. Another set of normal control group. After 12 weeks of drug intervention, retinal vessel morphology was observed by retina digestion method. The expression of VEGF protein was detected by Western blot. The expression of VEGF, Ras, Raf-1 and ERK mRNA was detected by Real-Time PCR. Results: Compared with the normal group, the area density of retinal capillaries and the ratio of endothelial cells / pericytes in model group were significantly increased (P <0.001), the expression of VEGF protein, VEGF, Ras and ERK mRNA increased (P <0.05) High (P> 0.05). Compared with the model group, the retinal capillary area density and the ratio of endothelial cells / pericytes in Huoxue Jiedu Decoction group decreased (P <0.01 and P <0.001), while the expression of VEGF, Ras, Raf-1 and ERK mRNA decreased 0.05). Conclusion: Huoxue Jiedu decoction can significantly inhibit the proliferation of retinal capillary and endothelial cells, and its mechanism may be through the intervention of Ras / Raf-1 / ERK signal transduction pathway, downregulation of VEGF expression in the retina, inhibition of angiogenesis, thereby improving DR .