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目的 :揭示杏仁体基底外侧核 (BL)中的γ 氨基丁酸 (GABA)能和乙酰胆碱能 (ACh) 2种中间神经元树突上的突触联系。方法 :用抗GABA和抗胆碱乙酰转移酶 (ChAT)抗体对BL做光镜和电镜免疫组化染色。结果 :光镜下 ,GABA免疫反应阳性神经元多为圆形多极神经元 ;ChAT免疫阳性神经元多为双极神经元。两者数量比约为 ( 7～ 9)∶1。电镜下 ,支配GABA免疫阳性神经元的突触 5 2 .7%为非对称性 (兴奋性 ) ,47.3 %为对称性 (抑制性 ) ,而ChAT免疫阳性神经元则分别为 44.9%和 5 5 .1 %。结论 :在情绪性学习记忆的处理过程中 ,杏仁体GABA能中间神经元起主要的功能作用 ,可能为抑制作用 ;而ACh能中间神经元作为辅助的均衡调节作用
Objective: To reveal the synaptic connections between GABA and ACh dendrites in the basolateral nucleus of amygdala (BL). Methods: Anti-GABA and anticholinergic acetyltransferase (ChAT) antibodies were used to make BL and light microscope and electron microscopy immunohistochemical staining. Results: Under light microscope, GABA immunoreactive neurons were mostly round multipolar neurons; ChAT immunoreactive neurons mostly bipolar neurons. The ratio between the two is about (7 ~ 9): 1. Under electron microscopy, synapses that dominate GABA-immunopositive neurons were 52.7% asymmetric (excitatory) and 47.3% symmetrical (inhibitory), while ChAT-immunoreactive neurons were 44.9% and 55%, respectively .1 %. Conclusion: In the processing of emotional learning and memory, alginate GABAergic interneurons play a major functional role, which may be an inhibitory effect. The ACh interneurons serve as an adjunct to the balanced regulation